| Title: |
Function of L-Pipecolic Acid as Compatible Solute in Corynebacterium glutamicum as Basis for Its Production Under Hyperosmolar Conditions |
| Authors: |
Fernando Pérez-García, Luciana F. Brito, Volker F. Wendisch |
| Source: |
Frontiers in Microbiology, Vol 10 (2019) |
| Publisher Information: |
Frontiers Media S.A., 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
pipecolic acid, osmo regulation, compatible solute, proline, Corynebacterium glutamicum, RNAseq analysis, Microbiology, QR1-502 |
| More Details: |
Pipecolic acid or L-PA is a cyclic amino acid derived from L-lysine which has gained interest in the recent years within the pharmaceutical and chemical industries. L-PA can be produced efficiently using recombinant Corynebacterium glutamicum strains by expanding the natural L-lysine biosynthetic pathway. L-PA is a six-membered ring homolog of the five-membered ring amino acid L-proline, which serves as compatible solute in C. glutamicum.Here, we show that de novo synthesized or externally added L-PA partially is beneficial for growth under hyper-osmotic stress conditions. C. glutamicum cells accumulated L-PA under elevated osmotic pressure and released it after an osmotic down shock. In the absence of the mechanosensitive channel YggB intracellular L-PA concentrations increased and its release after osmotic down shock was slower. The proline permease ProP was identified as a candidate L-PA uptake system since RNAseq analysis revealed increased proP RNA levels upon L-PA production. Under hyper-osmotic conditions, a ΔproP strain showed similar growth behavior than the parent strain when L-proline was added externally. By contrast, the growth impairment of the ΔproP strain under hyper-osmotic conditions could not be alleviated by addition of L-PA unless proP was expressed from a plasmid. This is commensurate with the view that L-proline can be imported into the C. glutamicum cell by ProP and other transporters such as EctP and PutP, while ProP appears of major importance for L-PA uptake under hyper-osmotic stress conditions. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00340/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2019.00340 |
| Access URL: |
https://doaj.org/article/6da1a0ce92ae4658a272cb8317d49ed0 |
| Accession Number: |
edsdoj.6da1a0ce92ae4658a272cb8317d49ed0 |
| Database: |
Directory of Open Access Journals |
