| Title: |
Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin |
| Authors: |
Asghar Narmani, Saeid Ganji, Maryam Amirishoar, Fatemeh Hajikarimi, Aynaz Mazandarani, Mahya Karimi, Ali Mohammadinejad, Azin Azadpour, Roghayyeh Jahedi, Elham Assadpour, Seid Mahdi Jafari |
| Source: |
Carbohydrate Polymer Technologies and Applications, Vol 9, Iss , Pp 100695- (2025) |
| Publisher Information: |
Elsevier, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Biochemistry |
| Subject Terms: |
PLGA-Chitosan nanocarrier, Dual targeting, Co-delivery, Controlled drug release, Breast cancer suppression, Apoptosis, Biochemistry, QD415-436 |
| More Details: |
Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC50 = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-8939 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666893925000349; https://doaj.org/toc/2666-8939 |
| DOI: |
10.1016/j.carpta.2025.100695 |
| Access URL: |
https://doaj.org/article/a6d877e485f0457989441ce747048992 |
| Accession Number: |
edsdoj.6d877e485f0457989441ce747048992 |
| Database: |
Directory of Open Access Journals |
