Bibliographic Details
| Title: |
Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses |
| Authors: |
Peipei Wu, Jihu Lu, Xuehua Zhang, Mei Mei, Lei Feng, Daxin Peng, Jibo Hou, Sang-Moo Kang, Xiufan Liu, Yinghua Tang |
| Source: |
Frontiers in Immunology, Vol 8 (2017) |
| Publisher Information: |
Frontiers Media S.A., 2017. |
| Publication Year: |
2017 |
| Collection: |
LCC:Immunologic diseases. Allergy |
| Subject Terms: |
H5 subtype influenza virus, vaccine, consensus, virus-like particles, broad protection, Immunologic diseases. Allergy, RC581-607 |
| More Details: |
The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-γ and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses. The rHA VLPs vaccine was superior to the commercial Re6 vaccine in conferring cross-protection against different clades of H5 subtype viruses. This study reports that H5 subtype consensus HA VLP single dose vaccination provides broad protection against HPAI virus in chickens. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-3224 |
| Relation: |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01649/full; https://doaj.org/toc/1664-3224 |
| DOI: |
10.3389/fimmu.2017.01649 |
| Access URL: |
https://doaj.org/article/6d7234584ce441cd8d286c207a175903 |
| Accession Number: |
edsdoj.6d7234584ce441cd8d286c207a175903 |
| Database: |
Directory of Open Access Journals |
