Bibliographic Details
| Title: |
Cancer-associated fibroblasts promote proliferation and drug resistance of gastric cancer organoids: a primary study |
| Authors: |
ZHANG Yuanyuan, DUAN Zhenquan, LI Yuxian |
| Source: |
陆军军医大学学报, Vol 47, Iss 5, Pp 453-461 (2025) |
| Publisher Information: |
Editorial Office of Journal of Army Medical University, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Medicine (General) |
| Subject Terms: |
gastric cancer, organoids, fibroblasts, co-culture model, drug sensitivity testing, Medicine (General), R5-920 |
| More Details: |
Objective To construct an in vitro co-culture model of gastric cancer organoids and cancer-associated fibroblasts (CAFs), and investigate the role of cancer-associated fibroblasts in the proliferation and chemotherapy resistance of gastric cancer organoids. Methods Tumor tissues from 12 gastric cancer patients undergoing surgical treatment in Department of General Surgery of Second Affiliated Hospital of Army Medical University from February 2023 to March 2024 were collected to construct gastric cancer organoids using 3D culture. HE staining was used to observe the morphology, and immunohistochemical assay was employed to determine the expression of cytokeratin CK7, carcinoembryonic antigen (CEA), and proliferation marker Ki-67. After CAFs derived from the same patient were cultured, observed for their morphology under a light microscope, and detected for the phenotype by flow cytometry, the cells were co-cultured with gastric cancer organoids in a 1:1 ratio. Phase-contrast microscopy was applied to observe the growth of the organoids and analyze the number, average diameter, and total area. Then, organoids cultured alone served as the control group. After the control and co-culture groups were treated with chemotherapy drugs, 5-fluorouracil and oxaliplatin, for 48 h, the viability and apoptosis of organoids were assessed with CellTiter-Glo 3D assay and CellEvent™ Caspase 3/7 activity, respectively. Results Gastric cancer organoids and CAFs were successfully established from 10 gastric cancer patient-derived samples. The gastric cancer organoids exhibited morphological characteristics consistent with the corresponding primary tumors, and showed positive expression of CK7, CEA, and Ki-67. CAFs displayed typical spindle-shaped morphology and exhibited the phenotypic markers CD326-, CD45-, CD31-, α-SMA+, CD73+, CD90+, and CD105+. Compared to the organoids cultured alone, the organoids co-cultured with CAFs showed more formation of organoids, in larger average diameter, and taking larger total area (P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
2097-0927 |
| Relation: |
https://aammt.tmmu.edu.cn/html/202410075.html; https://doaj.org/toc/2097-0927 |
| DOI: |
10.16016/j.2097-0927.202410075 |
| Access URL: |
https://doaj.org/article/6d10d441a79546fabedb9736bd5ed530 |
| Accession Number: |
edsdoj.6d10d441a79546fabedb9736bd5ed530 |
| Database: |
Directory of Open Access Journals |
