Experimental use of mycobacterial antigens in tuberculin skin test on water buffalo
| Title: | Experimental use of mycobacterial antigens in tuberculin skin test on water buffalo |
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| Authors: | Alessandra Martucciello, Federica Signorelli, Piera Mazzone, Francesco Grandoni, Giovanna Cappelli, Lucia Boccia, Giuseppe Parente, Monica Cagiola, Maria Beatrice Boniotti, Esterina De Carlo, Francesco Napolitano, Giorgio Galiero |
| Source: | Revista Científica, Vol 33, Iss Suplemento (2023) |
| Publisher Information: | Universidad del Zulia, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Cattle LCC:Veterinary medicine |
| Subject Terms: | water buffalo, tuberculosis, diagnosis, tuberculin skin test, Cattle, SF191-275, Veterinary medicine, SF600-1100 |
| More Details: | In Italy, bovine tuberculosis (TB) control in the water buffalo population is mainly carried out using the tuberculin skin test (TST). The test measures the increase in skin thickness 72 hours (h) after the intradermal injection of mycobacterial purified protein derivatives (PPD). The TST can involve the single intradermal test with bovine PPD (PPDB), or the comparative test adding a second injection with avian tuberculin (PPDA), using the couple of PPDs reduces false positives caused by non-tuberculosis mycobacteria. This pilot study aimed to evaluate the potential use of PPDB, PPDA, along with 6 kDa early secretory antigenic target (ESAT-6), 10 kDa culture filtrate protein (CFP-10), and Early Secretory Proteins (ESP) in the TST, in healthy and naturally TB infected buffaloes. Twenty-one buffaloes, gamma-interferon (IFN-γ) test positive, from a TB-out-break herd and 11 buffaloes from a TB-free herd were selected. All subjects were also submitted to the IFN-γ test using the same antigens (Ag) as the TST to verify the cellular response in vitro and to exclude anaergic animals that did not respond to in vitro stimulation with the mitogen. The TST was performed according to the TB Italian National eradication program, and to Reg. EU 2020/689. Eight intradermal injection sites were used on each animal shoulder (4 on the right and 4 on the left side). Three concentrations of the ESAT6-CFP10 protein cocktail (10, 20 and 30 μg) and two of ESP (50 and 100 μg) were inoculated. PPDB and PPDA were always included in the TST, along with PBS as negative control. Skin reactions were measured with calipers before the test and every 24 h for 4 days. The TST was considered positive in animals with an increase in skin thickness between post and pre-dermal test readings of > 4 mm, doubtful if between 2 and 4 mm, negative |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Spanish; Castilian |
| ISSN: | 0798-2259 2521-9715 |
| Relation: | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43384; https://doaj.org/toc/0798-2259; https://doaj.org/toc/2521-9715 |
| DOI: | 10.52973/rcfcv-wbc084 |
| Access URL: | https://doaj.org/article/6d0ec50af49b4ca2bdad0f025686ac6e |
| Accession Number: | edsdoj.6d0ec50af49b4ca2bdad0f025686ac6e |
| Database: | Directory of Open Access Journals |
| ISSN: | 07982259 25219715 |
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| DOI: | 10.52973/rcfcv-wbc084 |
| Published in: | Revista Científica |
| Language: | English Spanish; Castilian |