circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis
| Title: | circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis |
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| Authors: | Liyuan Yu, Kai Xia, Jing Zhou, Zhiai Hu, Xing Yin, Chenchen Zhou, Shujuan Zou, Jun Liu |
| Source: | International Journal of Oral Science, Vol 14, Iss 1, Pp 1-11 (2022) |
| Publisher Information: | Nature Publishing Group, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Dentistry |
| Subject Terms: | Dentistry, RK1-715 |
| More Details: | Abstract Human adipose-derived stem cells (hASCs) are a promising cell type for bone tissue regeneration. Circular RNAs (circRNAs) have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis. However, how circRNAs regulate hASCs in osteogenesis is still unclear. Herein, we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs. Knockdown of circ_0003204 by siRNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs. We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p. We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 mRNA. The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis. Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model, while overexpression of circ_0003204 inhibited bone defect repair. Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1674-2818 2049-3169 |
| Relation: | https://doaj.org/toc/1674-2818; https://doaj.org/toc/2049-3169 |
| DOI: | 10.1038/s41368-022-00184-2 |
| Access URL: | https://doaj.org/article/6cb36dd79852450b9b732e0cf869172d |
| Accession Number: | edsdoj.6cb36dd79852450b9b732e0cf869172d |
| Database: | Directory of Open Access Journals |
| ISSN: | 16742818 20493169 |
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| DOI: | 10.1038/s41368-022-00184-2 |
| Published in: | International Journal of Oral Science |
| Language: | English |