Bibliographic Details
| Title: |
Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG |
| Authors: |
Kirsten Alexandra Eberhardt, Felix Dewald, Eva Heger, Lutz Gieselmann, Kanika Vanshylla, Maike Wirtz, Franziska Kleipass, Wibke Johannis, Philipp Schommers, Henning Gruell, Karl August Brensing, Roman-Ulrich Müller, Max Augustin, Clara Lehmann, Manuel Koch, Florian Klein, Veronica Di Cristanziano |
| Source: |
Microorganisms, Vol 9, Iss 4, p 733 (2021) |
| Publisher Information: |
MDPI AG, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
pandemic, humoral response, neutralization, titer, protection, non-responder, Biology (General), QH301-705.5 |
| More Details: |
Background: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON® SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys® anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK® anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK® anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2076-2607 |
| Relation: |
https://www.mdpi.com/2076-2607/9/4/733; https://doaj.org/toc/2076-2607 |
| DOI: |
10.3390/microorganisms9040733 |
| Access URL: |
https://doaj.org/article/6c630c7a08bd4ce88b38dcb9bbf93f92 |
| Accession Number: |
edsdoj.6c630c7a08bd4ce88b38dcb9bbf93f92 |
| Database: |
Directory of Open Access Journals |
