Effects of Gliadin on Autoimmune Responses of Central Nervous System of C57BL/6 Mice
| Title: | Effects of Gliadin on Autoimmune Responses of Central Nervous System of C57BL/6 Mice |
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| Authors: | Parisa Esmaeili, Elham Rostami, Arefeh Akbarijavar, Alireza Akbari Meyestani, Hamed Bashiri, Mohammad Ali Rezaee, Shohreh Fakhari |
| Source: | Iranian Journal of Allergy, Asthma and Immunology, Vol 20, Iss 2 (2021) |
| Publisher Information: | Tehran University of Medical Sciences, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Medicine |
| Subject Terms: | Central nervous system, Gliadin, Immunity, Neurological disorder, Medicine |
| More Details: | Gluten sensitivity contributes to various degrees of neurological manifestations and neurodegenerative immunological changes. We investigated the experimental features of anti-gliadin immune responses in the central nervous system (CNS) of mice. Female C57BL6 mice were divided into three groups. Mice immunized with complete Freund's adjuvant (CFA) or gliadin emulsified in CFA, and the control group received phosphate-buffered saline (PBS). Immunohistochemistry, hematoxylin-eosin, and Luxol fast blue staining were performed on the sections. The serum levels of interleukin (IL)-17 and interferon-gamma (IFN-γ) were measured using enzyme-linked immunosorbent assay (ELISA). Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to assess the mRNA levels of chemokine (C-X-C motif) ligand-2 (CXCL-2), C-C motif chemokine ligand-2 (CCL-2), and CXCL-10. In gliadin+CFA immunized mice, the microscopic lesions included perivascular edema, focal-microgliosis, and acute neuronal necrosis in the cortex, subcortical, Purkinje cell layer, and ventral horn of the spinal cord. While extravasation of anti-IgG antibodies and selective targeting of Purkinje cells were observed in gliadin+CFA immunized mice. A significant increase in serum IL-17 and IFN-g levels (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1735-1502 1735-5249 |
| Relation: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2590; https://doaj.org/toc/1735-1502; https://doaj.org/toc/1735-5249 |
| DOI: | 10.18502/ijaai.v20i2.6052 |
| Access URL: | https://doaj.org/article/6c45b061b8644958b9096c9d4a1f00e4 |
| Accession Number: | edsdoj.6c45b061b8644958b9096c9d4a1f00e4 |
| Database: | Directory of Open Access Journals |
| ISSN: | 17351502 17355249 |
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| DOI: | 10.18502/ijaai.v20i2.6052 |
| Published in: | Iranian Journal of Allergy, Asthma and Immunology |
| Language: | English |