Protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative breast cancer xenograft model.

Bibliographic Details
Title:	Protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative breast cancer xenograft model.
Authors:	Maria K Tveitarås, Frode Selheim, Kristina Sortland, Rolf K Reed, Linda Stuhr
Source:	PLoS ONE, Vol 14, Iss 5, p e0215909 (2019)
Publisher Information:	Public Library of Science (PLoS), 2019.
Publication Year:	2019
Collection:	LCC:Medicine LCC:Science
Subject Terms:	Medicine, Science
More Details:	The main objective of this study was to identify single proteins or protein networks that might be used as diagnostic biomarkers or for therapeutic purposes by evaluating the protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative MDA-MB-231 breast cancer xenograft model. MDA-MB-231 tumour cells were injected into the mammary fat pads on one side of the groin area. The mice were sacrificed day 19 (pre-metastases) and day 54 (metastases). Non-injected mice served as controls. Plasma was collected and lungs harvested for both immunohistochemistry and protein analysis. The most striking observation in plasma was the initial reduction in haptoglobin level at the pre-metastatic stage, to a following significant increase in haptoglobin level at the metastatic stage, with a more than 4000-fold increase from the pre-metastatic to the metastatic phase. A corresponding increase in haptoglobin level was also found in lung tissue after metastasis. Fibrinogen beta chain also had a similar change in expression level in plasma as haptoglobin, however not as prominent. There were also changes in plasma thrombospondin-4 and transferrin receptor protein 1 levels, from an increase at the pre-metastatic stage, to a significant fall when metastases were established. This suggests that especially changes in haptoglobin, but also fibrinogen beta chain, thrombospondin-4 and transferrin receptor protein 1 is indicative of metastasis, at least in this breast cancer model, and should be further evaluated as general breast cancer biomarkers.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	1932-6203
Relation:	https://doaj.org/toc/1932-6203
DOI:	10.1371/journal.pone.0215909
Access URL:	https://doaj.org/article/6c178c7778f849c983e06370390d3ff3
Accession Number:	edsdoj.6c178c7778f849c983e06370390d3ff3
Database:	Directory of Open Access Journals
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More Details
ISSN:	19326203
DOI:	10.1371/journal.pone.0215909
Published in:	PLoS ONE
Language:	English