Academic Journal
Exploring cell-free assays for COVID-19 serosurvey
| Title: | Exploring cell-free assays for COVID-19 serosurvey |
|---|---|
| Authors: | Lucia Inchauste, Elif Nurtop, Nadège Brisbarre, Laetitia Ninove, Pierre Gallian, Xavier de Lamballerie, Stéphane Priet |
| Source: | Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024) |
| Publisher Information: | Nature Portfolio, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract Serosurveys to monitor immunity toward COVID-19 in the population are primarily performed using an ELISA to screen samples for SARS-CoV-2 antibodies, followed by confirmation by a virus neutralization test, which is considered the Gold Standard. However, virus neutralization test may not be feasible for some laboratories because of the requirement for specific facilities and trained personnel. In an attempt to address this limitation, we evaluated three cell-free methods as potential alternatives for assessing SARS-CoV-2 seroprevalence in human population from plasma. We report the establishment of two inhibition ELISAs designed to detect anti-Spike RBD IgG antibodies and a microsphere quantitative suspension array technology assay, based on the Luminex xMAP platform, to measure the presence of antibodies against various SARS-CoV-2 antigens, including anti-RBD. These methods were also compared to a commercial chemiluminescent immunoassay designed for anti-RBD antibodies detection and to the combined ELISA + virus neutralization test strategy. These cell-free assays performed equally to estimate the percentage of positive and negative samples and could be used to determine the prevalence of SARS-CoV-2 antibodies in human population, at least in cohort with high-expected prevalence, without the use of seroneutralization assay. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-55852-6 |
| Access URL: | https://doaj.org/article/6bb10027af0a4cdbb54b129c8ee10c4f |
| Accession Number: | edsdoj.6bb10027af0a4cdbb54b129c8ee10c4f |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
|---|---|
| DOI: | 10.1038/s41598-024-55852-6 |
| Published in: | Scientific Reports |
| Language: | English |