Bibliographic Details
| Title: |
Protective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation |
| Authors: |
Shuwen Li, Liangliang Li, Yijia Sun, Muhammad Zahoor Khan, Yue Yu, Lian Ruan, Li Chen, Juan Zhao, Junchi Jia, Yubao Li, Changfa Wang, Tongtong Wang |
| Source: |
Viruses, Vol 16, Iss 11, p 1765 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
cepharanthine, oxidative stress, EqHV-8, antiviral activity, AMPK/Nrf2/HO-1 signaling pathway, Microbiology, QR1-502 |
| More Details: |
Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses and donkeys, which is a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available to effectively treat EqHV-8 infection. Therefore, it is crucial to develop new antiviral approaches to prevent potential pandemics caused by EqHV-8. This study evaluates the antiviral and antioxidant effects of cepharanthine against EqHV-8 by employing both in vitro assays and in vivo mouse models to assess its therapeutic efficacy. To assess the effectiveness of cepharanthine against EqHV-8, we conducted experiments using NBL-6 and RK-13 cells. Additionally, we developed a mouse model to validate cepharanthine’s effectiveness against EqHV-8. In our in vitro experiments, we assessed the cepharanthine’s ability to inhibit infection caused by EqHV-8 in NBL-6 and RK-13 cells. Our results demonstrated that cepharanthine has a dose-dependent inhibitory effect, indicating that it possesses anti-EqHV-8 properties at the cellular level. Moreover, we investigated the mechanism through which cepharanthine exerts its protective effects. It was observed that cepharanthine effectively reduces the oxidative stress induced by EqHV-8 by activating the AMPK and Nrf2/HO-1 signaling pathways. Furthermore, when administered to EqHV-8 infected mice, cepharanthine significantly improved lung tissue pathology and reduced oxidative stress. The findings presented herein collectively highlight cepharanthine as a promising candidate for combating EqHV-8 infections. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1999-4915 |
| Relation: |
https://www.mdpi.com/1999-4915/16/11/1765; https://doaj.org/toc/1999-4915 |
| DOI: |
10.3390/v16111765 |
| Access URL: |
https://doaj.org/article/6b74852420f0486c868bbfb2d71d7bd8 |
| Accession Number: |
edsdoj.6b74852420f0486c868bbfb2d71d7bd8 |
| Database: |
Directory of Open Access Journals |
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