Bibliographic Details
| Title: |
miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| Authors: |
WANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan |
| Source: |
Jichu yixue yu linchuang, Vol 42, Iss 8, Pp 1230-1236 (2022) |
| Publisher Information: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
osteosarcoma, mir-499, tgf-α, saos2, Medicine |
| More Details: |
Objective To explore the role of miR-499 targeting TGF-α mRNA protein in regulating the migration and apoptosis of human osteosarcoma cell line Saos2 and its pathogenesis. Methods The expression differences of miR-499 and TGF-α in osteosarcoma tissues were detected by RT-qPCR. Double luciferase reporter gene assay was used to detect the targeting relationship between miR-499 and TGF-α. Transwell cell assay and TUNEL apoptosis assay were used to detect the migration and apoptosis of Saos2 cells. A nude mouse xenograft model of osteosarcoma was constructed to detect the regulatory mechanism of miR-499/TGF-α axis in vivo. Results In osteosarcoma, miR-499 expression was decreased (P＜0.01) but TGF-α mRNA expression was increased(P＜0.01). miR-499 negatively regulated TGF-α mRNA protein expression (P＜0.01). Transfection of miR-499 simulacrum inhibited the migration and promoted apoptosis of Saos2 cells (P＜0.01), while TGF-α promoted the migration and inhibited apoptosis of Saos2 cells (P＜0.01). miR-499 inhibited the carcinogenic effect of TGF-α. Conclusions miR-499 inhibits the biological function of osteosarcoma cell line Saos2 by inhibiting at TGF-α mRNA protein level which suggests that miR-499 may inhibit the development of osteosarcoma. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1001-6325 |
| Relation: |
http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-8-1230.pdf; https://doaj.org/toc/1001-6325 |
| DOI: |
10.16352/j.issn.1001-6325.2022.08.1230 |
| Access URL: |
https://doaj.org/article/d6b3797e92d1420f8e0939b061fc1c99 |
| Accession Number: |
edsdoj.6b3797e92d1420f8e0939b061fc1c99 |
| Database: |
Directory of Open Access Journals |
