Academic Journal
Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica
| Title: | Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica |
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| Authors: | Xiaoqin Liu, Jingyu Deng, Jinhong Zhang, Zhiyong Cui, Qingsheng Qi, Jin Hou |
| Source: | Biotechnology for Biofuels and Bioproducts, Vol 17, Iss 1, Pp 1-11 (2024) |
| Publisher Information: | BMC, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Biotechnology LCC:Fuel |
| Subject Terms: | Gene regulatory library, Non-homologous end joining, Targets identification, Yarrowia lipolytica, Biotechnology, TP248.13-248.65, Fuel, TP315-360 |
| More Details: | Abstract Background Genome-scale screening can be applied to efficiently mine for unknown genes with phenotypes of interest or special functions. It is also useful to identify new targets for engineering desirable properties of cell factories. Results Here, we designed a new approach for genome-scale transcription activation using non-homologous end joining (NHEJ)-mediated integration in Yarrowia lipolytica. We utilized this approach to screen for genes that, upon activation, confer phenotypes including improved acetic acid tolerance and xylose metabolism. The candidates were validated using gene overexpression, and functional changes including improved growth performance under multiple stressors and activated pentose metabolism were identified. Conclusions This study provides a simple and effective approach to randomly activate endogenous genes and mine for key targets associated with phenotypes of interest. The specific gene targets identified here will be useful for cell factory construction and biorefining lignocellulose. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2731-3654 |
| Relation: | https://doaj.org/toc/2731-3654 |
| DOI: | 10.1186/s13068-024-02472-x |
| Access URL: | https://doaj.org/article/ddd698e7aae44bfbbade402ca7afc93a |
| Accession Number: | edsdoj.698e7aae44bfbbade402ca7afc93a |
| Database: | Directory of Open Access Journals |
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| ISSN: | 27313654 |
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| DOI: | 10.1186/s13068-024-02472-x |
| Published in: | Biotechnology for Biofuels and Bioproducts |
| Language: | English |