Effect of Qingjie Fuzheng Granules on Proliferation, Apoptosis and Migration of Colorectal Cancer SW620 Cells
| Title: | Effect of Qingjie Fuzheng Granules on Proliferation, Apoptosis and Migration of Colorectal Cancer SW620 Cells |
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| Authors: | Xiaoqin ZHU, Hong YANG, Bin HUANG, Lihui WEI, Jiumao LIN |
| Source: | 康复学报, Vol 30, Pp 46-51 (2020) |
| Publisher Information: | Editorial Office of Rehabilitation Medicine, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine |
| Subject Terms: | colorectal cancer, Qingjie Fuzheng Granules (QFG), proliferation, apoptosis, migration, Medicine |
| More Details: | Objective:To investigate the effects and mechanisms of Qingjie Fuzheng Granules (QFG) on colorectal cancer SW620 cells in proliferation, apoptosis and migration.Methods:Colorectal cancer SW620 cells in vitro were treated with 0,0.5,1 and 2 mg/mL QFG. The inhibitory effect of QFG on cell proliferation was detected by MTT assay after 24 h and 48 h. The morphology of SW620 cells was observed with inverted microscope after 24 h. The cell cycle after 24 h was measured by flow cytometry. Apoptosis of SW620 cells after 24 h was detected by Hoechst 33258 staining test. The migration ability of cells after 0 h,6 h,12 h and 24 h was detected by scratching test. The expressions of Ki-67, MMP-2 and Cleaved-Caspase-3 proteins were detected by Western blot assay after 24 h.Results:(1) In the concentration range of 0,0.5,1,2 mg/mL, QFG significantly reduced the cell proliferation in a time and dose-dependent manner. The differences were statistically significant when compared with the control group in the same time (PPPP |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Chinese |
| ISSN: | 2096-0328 |
| Relation: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2020.01010; https://doaj.org/toc/2096-0328 |
| DOI: | 10.3724/SP.J.1329.2020.01010 |
| Access URL: | https://doaj.org/article/6879681471794026a9b3683c9515063d |
| Accession Number: | edsdoj.6879681471794026a9b3683c9515063d |
| Database: | Directory of Open Access Journals |
| ISSN: | 20960328 |
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| DOI: | 10.3724/SP.J.1329.2020.01010 |
| Published in: | 康复学报 |
| Language: | English Chinese |