| Title: |
Clinical utility of a commercial LAM-ELISA assay for TB diagnosis in HIV-infected patients using urine and sputum samples. |
| Authors: |
Keertan Dheda, Virginia Davids, Laura Lenders, Teri Roberts, Richard Meldau, Daphne Ling, Laurence Brunet, Richard van Zyl Smit, Jonathan Peter, Clare Green, Motasim Badri, Leonardo Sechi, Surendra Sharma, Michael Hoelscher, Rodney Dawson, Andrew Whitelaw, Jonathan Blackburn, Madhukar Pai, Alimuddin Zumla |
| Source: |
PLoS ONE, Vol 5, Iss 3, p e9848 (2010) |
| Publisher Information: |
Public Library of Science (PLoS), 2010. |
| Publication Year: |
2010 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
The accurate diagnosis of TB in HIV-infected patients, particularly with advanced immunosuppression, is difficult. Recent studies indicate that a lipoarabinomannan (LAM) assay (Clearview-TB(R)-ELISA) may have some utility for the diagnosis of TB in HIV-infected patients; however, the precise subgroup that may benefit from this technology requires clarification. The utility of LAM in sputum samples has, hitherto, not been evaluated.LAM was measured in sputum and urine samples obtained from 500 consecutively recruited ambulant patients, with suspected TB, from 2 primary care clinics in South Africa. Culture positivity for M. tuberculosis was used as the reference standard for TB diagnosis.Of 440 evaluable patients 120/387 (31%) were HIV-infected. Urine-LAM positivity was associated with HIV positivity (p = 0.007) and test sensitivity, although low, was significantly higher in HIV-infected compared to uninfected patients (21% versus 6%; p200 cells/mm(3) (37% versus 0%; p = 0.003). Urine-LAM remained highly specific in all 3 subgroups (95%-100%). 25% of smear-negative but culture-positive HIV-infected patients with a CD4 |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC2844421?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0009848 |
| Access URL: |
https://doaj.org/article/670128ab3cc74ad0ba8b1ed316592365 |
| Accession Number: |
edsdoj.670128ab3cc74ad0ba8b1ed316592365 |
| Database: |
Directory of Open Access Journals |
