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An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro

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Title: An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro
Authors: Alejandro Fuentes-Iglesias, Vera Garcia-Outeiral, Jose Angel Pardavila, Jianlong Wang, Miguel Fidalgo, Diana Guallar
Source: STAR Protocols, Vol 1, Iss 2, Pp 100093- (2020)
Publisher Information: Elsevier, 2020.
Publication Year: 2020
Collection: LCC:Science (General)
Subject Terms: Science (General), Q1-390
More Details: Summary: RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applications.For complete details on the use and execution of this protocol, please refer to Guallar et al. (2020).
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2666-1667
Relation: http://www.sciencedirect.com/science/article/pii/S2666166720300800; https://doaj.org/toc/2666-1667
DOI: 10.1016/j.xpro.2020.100093
Access URL: https://doaj.org/article/66f87f956af94b8b81d07f6132fadaeb
Accession Number: edsdoj.66f87f956af94b8b81d07f6132fadaeb
Database: Directory of Open Access Journals
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  Data: Summary: RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applications.For complete details on the use and execution of this protocol, please refer to Guallar et al. (2020).
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