Bibliographic Details
| Title: |
An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro |
| Authors: |
Alejandro Fuentes-Iglesias, Vera Garcia-Outeiral, Jose Angel Pardavila, Jianlong Wang, Miguel Fidalgo, Diana Guallar |
| Source: |
STAR Protocols, Vol 1, Iss 2, Pp 100093- (2020) |
| Publisher Information: |
Elsevier, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Science (General), Q1-390 |
| More Details: |
Summary: RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess the direct or indirect RNA-binding ability of any protein of interest. The versatility of this method relies on the adaptability of the immunoprecipitation conditions and the choice of the RNA, which exponentially broadens the range of potential applications.For complete details on the use and execution of this protocol, please refer to Guallar et al. (2020). |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166720300800; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100093 |
| Access URL: |
https://doaj.org/article/66f87f956af94b8b81d07f6132fadaeb |
| Accession Number: |
edsdoj.66f87f956af94b8b81d07f6132fadaeb |
| Database: |
Directory of Open Access Journals |
