Bibliographic Details
| Title: |
Schisandrin protects against ulcerative colitis by inhibiting the SGK1/NLRP3 signaling pathway and reshaping gut microbiota in mice |
| Authors: |
Xiaohu Wang, Chaozhuang Shen, Xingwen Wang, Jin Tang, Zijing Wu, Yunzhe Huang, Wenxin Shao, Kuo Geng, Haitang Xie, Zhichen Pu |
| Source: |
Chinese Medicine, Vol 18, Iss 1, Pp 1-16 (2023) |
| Publisher Information: |
BMC, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Other systems of medicine |
| Subject Terms: |
Schisandra chinensis (Turcz.) Baill., Schisandrin, Ulcerative colitis, NLRP3, Gut microbiota, Other systems of medicine, RZ201-999 |
| More Details: |
Abstract Background According to the Chinese Pharmacopoeia, the fruit of Schisandra chinensis (Turcz.) Baill. (SC) is an important traditional Chinese medicine that can be used to treat diarrhea. Despite the increasing research on the anti-inflammatory and anti-oxidant aspects of SC, the studies on the anti-ulcerative colitis of Schisandrin (SCH), the main constituent of SC, are relatively few. Methods The mice used in the study were randomly distributed into 6 groups: control, model, 5-ASA, and SCH (20, 40, 80 mg/kg/d). The mice in the model group were administered 3% (w/v) dextran sulfate sodium (DSS) through drinking water for 7 days, and the various parameters of disease activity index (DAI) such as body weight loss, stool consistency, and gross blood were measured. ELISA was used to detect inflammatory factors, and bioinformatics combined with transcriptome analysis was done to screen and verify relevant targets. 16S rDNA high-throughput sequencing was used to analyze the composition of the gut microbiota(GM), while mass spectrometry was done to analyze the changes in the content of bile acids (BAs) in the intestine. Results Mice treated with SCH experienced significant weight gain, effectively alleviating the severity of colitis, and decreasing the levels of inflammatory factors such as TNF-α, IL-1β, IL-18, IL-6, and other related proteins (NLRP3, Caspase-1, SGK1) in UC mice. Furthermore, the analysis of GM and BAs in mice revealed that SCH increased the relative abundance of Lactobacilli spp, reduced the relative abundance of Bacteroides, and promoted the conversion of primary BAs to secondary BAs. These effects contributed to a significant improvement in the DSS-induced GM imbalance and the maintenance of intestinal homeostasis. Conclusion It seems that there is a close relationship between the SCH mechanism and the regulation of SGK1/NLRP3 pathway and the restoration of GM balance. Therefore, it can be concluded that SCH could be a potential drug for the treatment of UC. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1749-8546 |
| Relation: |
https://doaj.org/toc/1749-8546 |
| DOI: |
10.1186/s13020-023-00815-8 |
| Access URL: |
https://doaj.org/article/66c672a6e946411ba2233686a1b82293 |
| Accession Number: |
edsdoj.66c672a6e946411ba2233686a1b82293 |
| Database: |
Directory of Open Access Journals |
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