Bibliographic Details
| Title: |
Modelling of the Citric Acid Production from Crude Glycerol by Wild-Type Yarrowia lipolytica DSM 8218 Using Response Surface Methodology (RSM) |
| Authors: |
Romina Giacomobono, Roberto Albergo, Vito Valerio, Antonio Caporusso, Isabella De Bari |
| Source: |
Life, Vol 12, Iss 5, p 621 (2022) |
| Publisher Information: |
MDPI AG, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Science |
| Subject Terms: |
bioconversion, oleaginous yeast, biorefinery, citric acid, crude glycerol, Response Surface Methodology, Science |
| More Details: |
Crude glycerol is the main by-product of the biodiesel manufacturing industry (10% w/w). Its use as a substrate in microbial fermentations is a concrete strategy to efficiently address its market surplus. In this study, the conversion of crude glycerol to citric acid, a key biochemical in the emerging bioeconomy, by a wild-type yeast Yarrowia lipolytica DSM 8218 was modelled using the Response Surface Methodology. The model relates C/N mass ratio and crude glycerol concentration to maximize the citric acid yield in flask scale using two different N sources, yeast extract and ammonium sulphate. Under the optimal conditions (yeast extract, C/N 141, glycerol 33 g/L), the conversion yield was 0.249 g/g. The optimal conditions were used for up-scaling a fed-batch fermentation in a 2 L bioreactor highlighting a metabolic shift from mannitol to citric acid when high stirring rates were applied (800 rpm). In these conditions, a morphic transition from pseudo-mycelial form to round-shaped yeast-like cells was observed too. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2075-1729 |
| Relation: |
https://www.mdpi.com/2075-1729/12/5/621; https://doaj.org/toc/2075-1729 |
| DOI: |
10.3390/life12050621 |
| Access URL: |
https://doaj.org/article/6614fc56977349dd9f44b73404c97835 |
| Accession Number: |
edsdoj.6614fc56977349dd9f44b73404c97835 |
| Database: |
Directory of Open Access Journals |
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