Bibliographic Details
| Title: |
A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis |
| Authors: |
Pengtao Gong, Xianhe Li, Wei Wu, Lili Cao, Panpan Zhao, Xin Li, Baoyan Ren, Jianhua Li, Xichen Zhang |
| Source: |
Frontiers in Microbiology, Vol 11 (2020) |
| Publisher Information: |
Frontiers Media S.A., 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Giardia duodenalis, Giardiavirus, microRNA, coding region, rdrp gene, Microbiology, QR1-502 |
| More Details: |
Giardia duodenalis is an important zoonotic parasite that can cause human and animal diarrhea. Giardiavirus (GLV) is a double-stranded RNA virus in Totiviridae family, which specifically infects trophozoites of the primitive protozoan parasite G. duodenalis. However, the GLV infectious and the pathogenicity of the G. duodenalis still remain to be confirmed. The GLV genome is 6,277 bp, which encodes two proteins (Gag and Gag-Pol). The expression of Gag-Pol protein is regulated by a-1 ribosomal frameshift. In this report, we identified a novel microRNA (GLV miRNA1) from the GLV. Split ligation northern results showed that GLV miRNA1 is a special expression product of GLV, and the precursor was also identified by primer extension. Antisense sequence of the GLV miRNA1 could increase the copy number of virus in G. duodenalis. It suggests that GLV miRNA1 governs the copy number of Giardiavirus in G. duodenalis. Most importantly, the GLV miRNA1 lies at the translated region of the rdrp gene, which is the first case that microRNA locates in the translated region of a known protein. It may be implying a novel phenomenon for miRNA biogenesis. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2020.569412/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2020.569412 |
| Access URL: |
https://doaj.org/article/6575ab8173f644969a287da27decdcc5 |
| Accession Number: |
edsdoj.6575ab8173f644969a287da27decdcc5 |
| Database: |
Directory of Open Access Journals |
