Bibliographic Details
| Title: |
Establishment and characterization of CHC-X1: the third human combined hepatocellular–cholangiocarcinoma cell line |
| Authors: |
Huan Tang, Changpeng Chai, Xin Miao, Yuanhui Su, Cheng Yu, Jianfeng Yi, Zhengfeng Wang, Hui Zhang, Zhenjie Zhao, Linpei Wang, Wence Zhou, Hao Xu |
| Source: |
BMC Cancer, Vol 25, Iss 1, Pp 1-14 (2025) |
| Publisher Information: |
BMC, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
Combined hepatocellular-cholangiocarcinoma, Cell lines, Transplanted tumor, Drug sensitivity, Drug resistance, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
Abstract Combined hepatocellular-cholangiocarcinoma (cHCC-CCA) represents an uncommon variant of primary liver cancer. In recent years, its incidence rate has increased. Thus, it is essential to perform comprehensive investigations into cHCC-CCA to develop suitable treatment strategies. So far, only two cell lines (CLs) of this cancer type have been reported. More cHCC-CCA CLs need to be established for research purposes. In this investigation, we developed a stable cHCC-CCA CL, named CHC-X1. STR analysis confirmed that CHC-X1 is a new human cHCC-CCA CL. CHC-X1 is a complex karyotype. Its population doubling time is 50.72 h. Under suspended conditions, CHC-X1 can form tumor spheres and organoids in Matrigel. These cells exhibit sensitivity to paclitaxel while demonstrating resistance against oxaliplatin, gemcitabine, and 5-FU. After inoculation into NXG mice, CHC-X1 can quickly form subcutaneous transplant tumors, exhibiting a tumor establishment rate of 67%. Immunohistochemical staining showed that CHC-X1 is a tumor CL with both liver cell differentiation and bile duct cell differentiation characteristics. It may function as a useful model for identifying the origins of cHCC-CCA and the advancement of potential treatments. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2407 |
| Relation: |
https://doaj.org/toc/1471-2407 |
| DOI: |
10.1186/s12885-025-13876-9 |
| Access URL: |
https://doaj.org/article/64b42eb45fa54a7b8ccea6c7c00e9839 |
| Accession Number: |
edsdoj.64b42eb45fa54a7b8ccea6c7c00e9839 |
| Database: |
Directory of Open Access Journals |
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