Bibliographic Details
| Title: |
Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes |
| Authors: |
Fengqiu Diao, Holly Ironfield, Haojiang Luan, Feici Diao, William C. Shropshire, John Ewer, Elizabeth Marr, Christopher J. Potter, Matthias Landgraf, Benjamin H. White |
| Source: |
Cell Reports, Vol 10, Iss 8, Pp 1410-1421 (2015) |
| Publisher Information: |
Elsevier, 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of “coding introns” (i.e., introns between coding exons). Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted “plug-and-play” cassettes (called “Trojan exons”) that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette) transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2211-1247 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2211124715001011; https://doaj.org/toc/2211-1247 |
| DOI: |
10.1016/j.celrep.2015.01.059 |
| Access URL: |
https://doaj.org/article/ce64a40b9434450e8d54a6c670a29143 |
| Accession Number: |
edsdoj.64a40b9434450e8d54a6c670a29143 |
| Database: |
Directory of Open Access Journals |
