Bibliographic Details
| Title: |
Development of a Bioprocess for the Production of Cyclic Lipopeptides Pseudofactins With Efficient Purification From Collected Foam |
| Authors: |
Piotr Biniarz, Marius Henkel, Rudolf Hausmann, Marcin Łukaszewicz |
| Source: |
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
| Publisher Information: |
Frontiers Media S.A., 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Biotechnology |
| Subject Terms: |
biosurfactant production, cyclic lipopeptides (CLPs), Pseudomonas fluorescens, bioreactor, lipopeptide production, lipopeptide purification, Biotechnology, TP248.13-248.65 |
| More Details: |
Microbial surfactants (biosurfactants) have gained interest as promising substitutes of synthetic surface-active compounds. However, their production and purification are still challenging, with significant room for efficiency and costs optimization. In this work, we introduce a method for the enhanced production and purification of cyclic lipopeptides pseudofactins (PFs) from Pseudomonas fluorescens BD5 cultures. The method is directly applicable in a technical scale with the possibility of further upscaling. Comparing to the original protocol for production of PFs (cultures in mineral salt medium in shaken flasks followed by solvent-solvent extraction of PFs), our process offers not only ∼24-fold increased productivity, but also easier and more efficient purification. The new process combines high yield of PFs (∼7.2 grams of PFs per 30 L of working volume), with recovery levels of 80–90% and purity of raw PFs up to 60–70%. These were achieved with an innovative, single-step thermal co-precipitation and extraction of PFs directly from collected foam, as a large amount of PF-enriched foam was produced during the bioprocess. Besides we present a protocol for the selective production of PF structural analogs and their separation with high-performance liquid chromatography. Our approach can be potentially utilized in the efficient production and purification of other lipopeptides of Pseudomonas and Bacillus origin. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-4185 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fbioe.2020.565619/full; https://doaj.org/toc/2296-4185 |
| DOI: |
10.3389/fbioe.2020.565619 |
| Access URL: |
https://doaj.org/article/648af5cd57fc4145a9e52c4706a390f0 |
| Accession Number: |
edsdoj.648af5cd57fc4145a9e52c4706a390f0 |
| Database: |
Directory of Open Access Journals |
