Bibliographic Details
| Title: |
Dehydrated Human Amnion-Chorion Membrane Extracts Can Ameliorate Interstitial Cystitis in Rats by Down-Regulating Inflammatory Cytokines and Protein Coding Genes: A Preclinical Study |
| Authors: |
Che-Hsueh Yang, Min-Che Tung, Yi-Sheng Lin, Chao-Yu Hsu, Ivan Akhrymuk, Kok-Tong Tan, Yen-Chuan Ou, Chi-Chien Lin |
| Source: |
Life, Vol 12, Iss 11, p 1693 (2022) |
| Publisher Information: |
MDPI AG, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Science |
| Subject Terms: |
animal, anti-infective agents/therapeutic use, amnion/transplantation, cystitis, interstitial/therapy, chorion transplantation, Science |
| More Details: |
The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-µL lipopolysaccharide (1 µg/µL), and it was replaced with normal saline in the sham control group. After IC induction, 300 µL dehydrated HACM extracts (3 mg/kg) were instilled into rats’ urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-κB, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-α, IL-1β, IL-6, IL-8, and NF-κB were all down-regulated by dehydrated HACM extracts (p < 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (p < 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (p < 0.05) after instillation. In fibrosis, dysregulated TGFβ/R-Smads/Snail was corrected by the instillation of dehydrated HACM (p < 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2075-1729 |
| Relation: |
https://www.mdpi.com/2075-1729/12/11/1693; https://doaj.org/toc/2075-1729 |
| DOI: |
10.3390/life12111693 |
| Access URL: |
https://doaj.org/article/c645f009db81432281ba3b44fce1d112 |
| Accession Number: |
edsdoj.645f009db81432281ba3b44fce1d112 |
| Database: |
Directory of Open Access Journals |
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