Bibliographic Details
| Title: |
Anti-nociceptive Activity of Quebracho tannin Extract on Pain Induced by Formalin and Writhing Tests in Mice |
| Authors: |
Fatemeh Zare, Shahin Hassanpour, Ahmad Asghari, Alireza Jahandideh |
| Source: |
Avicenna Journal of Medical Biochemistry, Vol 9, Iss 2, Pp 59-64 (2021) |
| Publisher Information: |
Hamadan University of Medical Sciences, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Medical technology |
| Subject Terms: |
quebracho tannin, pain, formalin test, mice, Medical technology, R855-855.5 |
| More Details: |
Background: Based on positive role of the tannins for pain relief, there is no report for possible anti-nociceptive activity of the Quebracho tannin. Objectives: This study aimed to determine the anti-nociceptive activity of the Quebracho tannin extract (QTE) on pain in mice. Materials and Methods: For this purpose, 340 mice were used for formalin and writhing tests each including 4 experiments with 4 sub-groups. In experiment 1, mice were injected with saline, QTE (100 mg/kg), QTE (200 mg/kg), QTE (400 mg/kg), and morphine (5 mg/kg). In the second experiment, injections included saline, QTE (400 mg/kg), naloxone (2 mg/kg), and QTE + naloxone. Experiments 3 and 4 were similar to experiment 2, except that mice injected were with NG-nitro arginine methyl ester (L-NAME, 10 mg/kg) and cyproheptadine (4 mg/kg) instead of naloxone. Then, formalin (1%) was injected, and time spent for licking the injected paw was recorded until 30 minutes following injection in the first and second phases. Finally, injections in 4 experiment groups were the same, and animals were intraperitoneally injected with acetic acid, and contractions were recorded in the writhing test category. Results: According to the results, QTE (100, 200, and 400 mg/kg) decreased pain in the injected paw (P=0.001) and inhibited the pain response by 59.37% (P=0.001). Moreover, the injection of naloxone + QTE significantly decreased pain in the injected paw (P=0.021). Eventually, the injection of the L-NAME + QTE significantly reduced the anti-nociception effect of the QTE on the formalin test (P=0.031) and writhing contractions (55.75%, P=0.033). Conclusion: These findings suggested anti-nociceptive properties of the QTE mediated by opioidergic and nitrergic systems. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2345-4113 |
| Relation: |
http://ajmb.umsha.ac.ir/PDF/ajmb-9-59.pdf; https://doaj.org/toc/2345-4113 |
| DOI: |
10.34172/ajmb.2021.11 |
| Access URL: |
https://doaj.org/article/63b2fc1bfe7a470cb18efc8f5290ee60 |
| Accession Number: |
edsdoj.63b2fc1bfe7a470cb18efc8f5290ee60 |
| Database: |
Directory of Open Access Journals |
