Bibliographic Details
| Title: |
Yeast protein‐derived γ‐glutamyl peptides prepared by transpeptidation reaction exhibit a pronounced taste‐enhancing effect |
| Authors: |
Jiaying Liu, Yu Fu, Liang Ma, Hongjie Dai, Hongxia Wang, Hai Chen, Hankun Zhu, Yong Yu, Xin Liu, Zhengfang Liu, Yuhao Zhang |
| Source: |
Food Frontiers, Vol 5, Iss 6, Pp 2782-2795 (2024) |
| Publisher Information: |
Wiley, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Nutrition. Foods and food supply
LCC:Food processing and manufacture |
| Subject Terms: |
mixed peptides taste‐enhancing effect, molecular docking, transpeptidation reaction, yeast protein, γ‐glutamyl peptides, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| More Details: |
Abstract A high‐salt diet can induce hypertension, so salt reduction can prevent hypertension. γ‐Glutamyl peptides (GGPs) have obvious taste‐enhancing effects, while their contents in natural foods are relatively low. Yeast protein rich in Glu/Gln is a good precursor for the preparation of GGPs. In this study, yeast protein‐derived GGPs were prepared through hydrolysis and transpeptidation reactions, followed by sensory evaluation and E‐tongue analysis. Peptide sequences were identified by LC−MS/MS and screened for molecular docking. The optimal reaction conditions were hydrolysis for 4 h, enzyme concentration of 16 U/g, and transpeptidation for 4 h. GGPs could increase salt and umami intensity by 60.78% and 40.93% based on sensory evaluation, 22.52%, and 16.40% according to E‐tongue analysis. Fifteen γ‐glutamyl peptides with different peptide lengths were selected for molecular docking. Molecular docking confirmed their binding to calcium‐sensing receptors (CaSr) through hydrogen bonds and hydrophobic interaction, while interaction between CaSR receptor and γ‐glutamyl di‐, tri‐, and oligo‐peptides varied in binding energy. The stimulation received by CaSR lasted a longer time and varied in intensity. It was further proved that the flavor of mixed peptides has a layered sense and can give people a rich taste experience. Overall, yeast protein‐derived GGPs can enhance salt and umami taste, which can reduce salt usage without compromising taste. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2643-8429 |
| Relation: |
https://doaj.org/toc/2643-8429 |
| DOI: |
10.1002/fft2.479 |
| Access URL: |
https://doaj.org/article/dd630feea2c54e2bb6b9f2942f005899 |
| Accession Number: |
edsdoj.630feea2c54e2bb6b9f2942f005899 |
| Database: |
Directory of Open Access Journals |
