Academic Journal
The WY Domain of an RxLr Effector Drives Interactions with a Host Target Phosphatase to Mimic Host Regulatory Proteins and Promote Phytophthora infestans Infection
| Title: | The WY Domain of an RxLr Effector Drives Interactions with a Host Target Phosphatase to Mimic Host Regulatory Proteins and Promote Phytophthora infestans Infection |
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| Authors: | Adam R. Bentham, Wei Wang, Franziska Trusch, Freya A. Varden, Paul R. J. Birch, Mark J. Banfield |
| Source: | Molecular Plant-Microbe Interactions, Vol 37, Iss 3, Pp 239-249 (2024) |
| Publisher Information: | The American Phytopathological Society, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Microbiology LCC:Botany |
| Subject Terms: | oomycete, Phytophthora infestans, plant–pathogen interactions, potato, protein–protein interactions, structural biology, Microbiology, QR1-502, Botany, QK1-989 |
| More Details: | Plant pathogens manipulate the cellular environment of the host to facilitate infection and colonization that often lead to plant diseases. To accomplish this, many specialized pathogens secrete virulence proteins called effectors into the host cell, which subvert processes such as immune signaling, gene transcription, and host metabolism. Phytophthora infestans, the causative agent of potato late blight, employs an expanded repertoire of RxLR effectors with WY domains to manipulate the host through direct interaction with protein targets. However, our understanding of the molecular mechanisms underlying the interactions between WY effectors and their host targets remains limited. In this study, we performed a structural and biophysical characterization of the P. infestans WY effector Pi04314 in complex with the potato Protein Phosphatase 1-c (PP1c). We elucidate how Pi04314 uses a WY domain and a specialized C-terminal loop carrying a KVxF motif that interact with conserved surfaces on PP1c, known to be used by host regulatory proteins for guiding function. Through biophysical and in planta analyses, we demonstrate that Pi04314 WY or KVxF mutants lose their ability to bind PP1c. The loss of PP1c binding correlates with changes in PP1c nucleolar localization and a decrease in lesion size in plant infection assays. This study provides insights into the manipulation of plant hosts by pathogens, revealing how effectors exploit key regulatory interfaces in host proteins to modify their function and facilitate disease. [Graphic: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1943-7706 0894-0282 80048331 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-08-23-0118-FI |
| Access URL: | https://doaj.org/article/62079bf8ec4842b68e800483319d6a1b |
| Accession Number: | edsdoj.62079bf8ec4842b68e800483319d6a1b |
| Database: | Directory of Open Access Journals |
| ISSN: | 19437706 08940282 80048331 |
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| DOI: | 10.1094/MPMI-08-23-0118-FI |
| Published in: | Molecular Plant-Microbe Interactions |
| Language: | English |