A cryo-electron tomography study of ciliary rootlet organization
| Title: | A cryo-electron tomography study of ciliary rootlet organization |
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| Authors: | Chris van Hoorn, Andrew P Carter |
| Source: | eLife, Vol 12 (2024) |
| Publisher Information: | eLife Sciences Publications Ltd, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine LCC:Science LCC:Biology (General) |
| Subject Terms: | photoreceptor cell, cilia, centriole, ciliary rootlet, membrane anchoring, retina, Medicine, Science, Biology (General), QH301-705.5 |
| More Details: | Ciliary rootlets are striated bundles of filaments that connect the base of cilia to internal cellular structures. Rootlets are critical for the sensory and motile functions of cilia. However, the mechanisms underlying these functions remain unknown, in part due to a lack of structural information of rootlet organization. In this study, we obtain 3D reconstructions of membrane-associated and purified rootlets from mouse retina using cryo-electron tomography. We show that flexible protrusions on the rootlet surface, which emanate from the cross-striations, connect to intracellular membranes. In purified rootlets, the striations were classified into amorphous (A)-bands, associated with accumulations on the rootlet surface, and discrete (D)-bands corresponding to punctate lines of density that run through the rootlet. These striations connect a flexible network of longitudinal filaments. Subtomogram averaging suggests the filaments consist of two intertwined coiled coils. The rootlet’s filamentous architecture, with frequent membrane-connecting cross-striations, lends itself well for anchoring large membranes in the cell. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2050-084X |
| Relation: | https://elifesciences.org/articles/91642; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.91642 |
| Access URL: | https://doaj.org/article/d61e778fe30e48f8b195088d39f0a4d8 |
| Accession Number: | edsdoj.61e778fe30e48f8b195088d39f0a4d8 |
| Database: | Directory of Open Access Journals |
| ISSN: | 2050084X |
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| DOI: | 10.7554/eLife.91642 |
| Published in: | eLife |
| Language: | English |