Bibliographic Details
| Title: |
Seed Dormancy Involves a Transcriptional Program That Supports Early Plastid Functionality during Imbibition |
| Authors: |
Alberto Gianinetti, Franca Finocchiaro, Paolo Bagnaresi, Antonella Zechini, Primetta Faccioli, Luigi Cattivelli, Giampiero Valè, Chiara Biselli |
| Source: |
Plants, Vol 7, Iss 2, p 35 (2018) |
| Publisher Information: |
MDPI AG, 2018. |
| Publication Year: |
2018 |
| Collection: |
LCC:Botany |
| Subject Terms: |
dry-afterripening, weedy rice, Oryza sativa, dormancy, germination, transcriptome, plastid, Botany, QK1-989 |
| More Details: |
Red rice fully dormant seeds do not germinate even under favorable germination conditions. In several species, including rice, seed dormancy can be removed by dry-afterripening (warm storage); thus, dormant and non-dormant seeds can be compared for the same genotype. A weedy (red) rice genotype with strong dormancy was used for mRNA expression profiling, by RNA-Seq, of dormant and non-dormant dehulled caryopses (here addressed as seeds) at two temperatures (30 °C and 10 °C) and two durations of incubation in water (8 h and 8 days). Aim of the study was to highlight the differences in the transcriptome of dormant and non-dormant imbibed seeds. Transcript data suggested important differences between these seeds (at least, as inferred by expression-based metabolism reconstruction): dry-afterripening seems to impose a respiratory impairment onto non-dormant seeds, thus glycolysis is deduced to be preferentially directed to alcoholic fermentation in non-dormant seeds but to alanine production in dormant ones; phosphoenolpyruvate carboxykinase, pyruvate phosphate dikinase and alanine aminotransferase pathways appear to have an important gluconeogenetic role associated with the restoration of plastid functions in the dormant seed following imbibition; correspondingly, co-expression analysis pointed out a commitment to guarantee plastid functionality in dormant seeds. At 8 h of imbibition, as inferred by gene expression, dormant seeds appear to preferentially use carbon and nitrogen resources for biosynthetic processes in the plastid, including starch and proanthocyanidins accumulation. Chromatin modification appears to be a possible mechanism involved in the transition from dormancy to germination. Non-dormant seeds show higher expression of genes related to cell wall modification, suggesting they prepare for acrospire/radicle elongation. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2223-7747 |
| Relation: |
http://www.mdpi.com/2223-7747/7/2/35; https://doaj.org/toc/2223-7747 |
| DOI: |
10.3390/plants7020035 |
| Access URL: |
https://doaj.org/article/619af21a792d4d178e95720598fdb0e0 |
| Accession Number: |
edsdoj.619af21a792d4d178e95720598fdb0e0 |
| Database: |
Directory of Open Access Journals |
