Bibliographic Details
| Title: |
Novel method for quantification of lipoprotein(a)-cholesterol: implications for improving accuracy of LDL-C measurements |
| Authors: |
Calvin Yeang, Joseph L. Witztum, Sotirios Tsimikas |
| Source: |
Journal of Lipid Research, Vol 62, Iss , Pp 100053- (2021) |
| Publisher Information: |
Elsevier, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Biochemistry |
| Subject Terms: |
lipoprotein(a), low density lipoprotein, cholesterol, biomarker, cardiovascular disease risk, therapy, Biochemistry, QD415-436 |
| More Details: |
Current methods for determining “LDL-C” in clinical practice measure the cholesterol content of both LDL and lipoprotein(a) [Lp(a)-C]. We developed a high-throughput, sensitive, and rapid method to quantitate Lp(a)-C and improve the accuracy of LDL-C by subtracting for Lp(a)-C (LDL-Ccorr). Lp(a)-C is determined following isolation of the Lp(a) on magnetic beads linked to monoclonal antibody LPA4 recognizing apolipoprotein(a). This Lp(a)-C assay does not detect cholesterol in plasma samples lacking Lp(a) and is linear up to 747 nM Lp(a). To validate this method clinically over a wide range of Lp(a) (9.0–822.8 nM), Lp(a)-C and LDL-Ccorr were determined in 21 participants receiving an Lp(a)-specific lowering antisense oligonucleotide and in eight participants receiving placebo at baseline, at 13 weeks during peak drug effect, and off drug. In the groups combined, Lp(a)-C ranged from 0.6 to 35.0 mg/dl and correlated with Lp(a) molar concentration (r = 0.76; P < 0.001). However, the percent Lp(a)-C relative to Lp(a) mass varied from 5.8% to 57.3%. Baseline LDL-Ccorr was lower than LDL-C [mean (SD), 102.2 (31.8) vs. 119.2 (32.4) mg/dl; P < 0.001] and did not correlate with Lp(a)-C. It was demonstrated that three commercially available “direct LDL-C” assays also include measures of Lp(a)-C. In conclusion, we have developed a novel and sensitive method to quantitate Lp(a)-C that provides insights into the Lp(a) mass/cholesterol relationship and may be used to more accurately report LDL-C and reassess its role in clinical medicine. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227521000341; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1016/j.jlr.2021.100053 |
| Access URL: |
https://doaj.org/article/615be8f28e424120957595e6d6cd41ff |
| Accession Number: |
edsdoj.615be8f28e424120957595e6d6cd41ff |
| Database: |
Directory of Open Access Journals |
