Bibliographic Details
| Title: |
Identification of Two R2R3-MYB Genes Involved in Flavan-3-Ols Biosynthesis as Modulated by Salicylic Acid Through RNA-Seq in Grape Berries (Vitis spp.) |
| Authors: |
Fengchan Yu, Pengfei Zhai, Wenjing Ge, Runtian Zhou, Shuwen Zan, Jinjun Liang, Pengfei Wen, Yu Gary Gao |
| Source: |
Agriculture, Vol 14, Iss 11, p 2069 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Agriculture (General) |
| Subject Terms: |
Vitis vinifera, salicylic acid, flavan-3-ols, RNA-seq, WGCNA, Agriculture (General), S1-972 |
| More Details: |
Flavan-3-ols are plant secondary metabolites that play important roles in stress resistance. Our previous studies revealed that salicylic acid (SA) activates R2R3-MYB transcription factors, promoting flavan-3-ol biosynthesis. This study identified two R2R3-MYB genes that exhibited positive responses to both exogenous SA and were probably involved in flavan-3-ol biosynthesis through RNA-sequencing, functional enrichment analysis, and qRT-PCR. The results indicated that the contents of total flavan-3-ols and their monomers, (+)-catechin and (−)-epicatechin, in grape berries after exogenous SA application were substantially increased compared to those in the control. A total of 683 differentially expressed genes in response to exogenous SA treatment were identified using RNA-seq. KEGG analysis revealed enrichment of the ‘flavonoid biosynthesis’ and ‘plant hormone signal transduction’ pathways. A specific module highly associated with flavan-3-ol biosynthesis was identified by constructing a co-expression network. Two candidate genes (VvMYB108B and VvMYB145) likely participating in flavan-3-ol biosynthesis were selected using qRT-PCR. Therefore, these two potential genes that respond to SA and putatively participate in flavan-3-ol biosynthesis were identified for the first time. These results lay a solid basis for a more profound understanding of the molecular regulation of flavan-3-ol biosynthesis in grapes. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2077-0472 |
| Relation: |
https://www.mdpi.com/2077-0472/14/11/2069; https://doaj.org/toc/2077-0472 |
| DOI: |
10.3390/agriculture14112069 |
| Access URL: |
https://doaj.org/article/614f8013acab470aa6167ef8ea0cf1cc |
| Accession Number: |
edsdoj.614f8013acab470aa6167ef8ea0cf1cc |
| Database: |
Directory of Open Access Journals |
