Academic Journal
Validation of RT-qPCR test for SARS-CoV-2 in saliva specimens
| Title: | Validation of RT-qPCR test for SARS-CoV-2 in saliva specimens |
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| Authors: | Luis Miguel Sosa Ávila, Martha Lucía Díaz Galvis, Mayra Alejandra Jaimes Campos, Anyela Lozano-Parra, Laura Andrea Rodríguez Villamizar, Myriam Oróstegui Arenas, Ruth Aralí Martínez-Vega, Lina María Vera Cala, Leonelo E. Bautista |
| Source: | Journal of Infection and Public Health, Vol 15, Iss 12, Pp 1403-1408 (2022) |
| Publisher Information: | Elsevier, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| Subject Terms: | Polymerase chain reaction, COVID-19 testing, SARS-CoV-2, Saliva, Latent class analysis, Bayes Theorem, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| More Details: | Background: Saliva samples may be an easier, faster, safer, and cost-saving alternative to NPS samples, and can be self-collected by the patient. Whether SARS-CoV-2 RT-qPCR in saliva is more accurate than in nasopharyngeal swaps (NPS) is uncertain. We evaluated the accuracy of the RT-qPCR in both types of samples, assuming both approaches were imperfect. Methods: We assessed the limit of detection (LoD) of RT-qPCR in each type of sample. We collected paired NPS and saliva samples and tested them using the Berlin Protocol to detect SARS-CoV-2 envelope protein (E). We used a Bayesian latent class analysis (BLCA) to estimate the sensitivity and specificity of each test, while accounting for their conditional dependence. Results: The LoD were 10 copies/mL in saliva and 100 copies/mL in NPS. Paired samples of saliva and NPS were collected in 412 participants. Out of 68 infected cases, 14 were positive only in saliva. RT-qPCR sensitivity ranged from 82.7 % (95 % CrI: 54.8, 94.8) in NPS to 84.5 % (50.9, 96.5) in saliva. Corresponding specificities were 99.1 % (95 % CrI: 95.3, 99.8) and 98.4 %(95 % CrI: 92.8, 99.7). Conclusions: SARS-CoV-2 RT-qPCR test in saliva specimens has a similar or better accuracy than RT-qPCR test in NPS. Saliva specimens may be ideal for surveillance in general population, particularly in children, and in healthcare or other personnel in need of serial testing. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1876-0341 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1876034122002933; https://doaj.org/toc/1876-0341 |
| DOI: | 10.1016/j.jiph.2022.10.028 |
| Access URL: | https://doaj.org/article/5f11fd9203294457918156d6b3781db9 |
| Accession Number: | edsdoj.5f11fd9203294457918156d6b3781db9 |
| Database: | Directory of Open Access Journals |
| ISSN: | 18760341 |
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| DOI: | 10.1016/j.jiph.2022.10.028 |
| Published in: | Journal of Infection and Public Health |
| Language: | English |