Th1-polarized MtrE-based gonococcal vaccines display prophylactic and therapeutic efficacy
| Title: | Th1-polarized MtrE-based gonococcal vaccines display prophylactic and therapeutic efficacy |
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| Authors: | Shuaijie Song, Shuyi Wang, Xiaoyun Jiang, Fan Yang, Shuai Gao, Xu’ai Lin, Hao Cheng, Stijn van der Veen |
| Source: | Emerging Microbes and Infections, Vol 12, Iss 2 (2023) |
| Publisher Information: | Taylor & Francis Group, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| Subject Terms: | Neisseria gonorrhoeae, vaccine, MtrE, CpG1826, Th1 polarization, FC428, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| More Details: | Global dissemination of high-level ceftriaxone-resistant Neisseria gonorrhoeae strains associated with the FC428 clone poses a threat to the efficacy ceftriaxone-based therapies. Vaccination is the best strategy to contain multidrug-resistant infections. In this study, we investigated the efficacy of MtrE and its surface Loop2 as vaccine antigens when combined with a Th1-polarizing adjuvant, which is expected to be beneficial for gonococcal vaccine development. Using in vitro dendritic cell maturation and T cell differentiation assays, CpG1826 was identified as the optimal Th1-polarizing adjuvant for MtrE and Loop2 displayed as linear epitope (Nloop2) or structural epitope (Intraloop2) on a carrier protein. Loop2-based antigens raised strongly Th1-polarized and bactericidal antibody responses in vaccinated mice. Furthermore, the vaccine formulations provided protection against a gonococcal challenge in mouse vaginal tract infection model when provided as prophylactic vaccines. Also, the vaccine formulations accelerated gonococcal clearance when provided as a single therapeutic dose to treat an already established infection, including against a strain associated with the FC428 clone. Therefore, this study demonstrated that MtrE and Loop 2 are effective gonococcal vaccine antigens when combined with the Th1-polarizing CpG1826 adjuvant. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2023.2249124 |
| Access URL: | https://doaj.org/article/c5d80acbb4774ec3a0c33594beb80971 |
| Accession Number: | edsdoj.5d80acbb4774ec3a0c33594beb80971 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22221751 |
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| DOI: | 10.1080/22221751.2023.2249124 |
| Published in: | Emerging Microbes and Infections |
| Language: | English |