Bibliographic Details
| Title: |
Single-Cell Sorting of HBsAg-Binding Memory B Cells from Human Peripheral Blood Mononuclear Cells and Antibody Cloning |
| Authors: |
Yunjiao Zhou, Zhenmi Liu, Zijun Wang, Qianqian Zhang, Christian T. Mayer, Till Schoofs, Michel C. Nussenzweig, Ype P. de Jong, Qiao Wang |
| Source: |
STAR Protocols, Vol 1, Iss 3, Pp 100129- (2020) |
| Publisher Information: |
Elsevier, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Science (General), Q1-390 |
| More Details: |
Summary: The isolation of human antibodies with naturally paired heavy and light chains is crucial for understanding the human antibody immune response. Here, we present a protocol for antibody cloning from the sorted single human memory B cells recognizing hepatitis B virus (HBV) S antigen (HBsAg). A two-fluorescent-dye labeling strategy against HBsAg allows for an improved sorting specificity, while non-relevant protein staining allows for the exclusion of non-specific B cells. This protocol could also be widely adapted for other antigens.For complete details on the use and execution of this protocol, please refer to Wang et al. (2020). |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166720301167; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100129 |
| Access URL: |
https://doaj.org/article/5d4ee08913df4a6786a8fbd2879d1303 |
| Accession Number: |
edsdoj.5d4ee08913df4a6786a8fbd2879d1303 |
| Database: |
Directory of Open Access Journals |
