Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water
| Title: | Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water |
|---|---|
| Authors: | Amer Jamal Hashmat, Muhammad Afzal, Samina Iqbal, Imran Amin, Carlos Alberto Arias, Hans Brix, Imran Zafar, Sania Riaz, Rizwan ur Rehman, Ahmad Mohammad Salamatullah, Gezahign Fentahun Wondmie, Mohammed Bourhia |
| Source: | Bioresources and Bioprocessing, Vol 11, Iss 1, Pp 1-8 (2024) |
| Publisher Information: | SpringerOpen, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Technology LCC:Chemical technology LCC:Biotechnology |
| Subject Terms: | Crude oil, Constructed wetlands, Bacterial persistence, Metabolic activity, Gene abundance and expression, Technology, Chemical technology, TP1-1185, Biotechnology, TP248.13-248.65 |
| More Details: | Abstract The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) alkB gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium (Pseudomonas putida TYRI39, Acinetobacter junii TYRH47, Acinetobacter sp. CYRH17, Pseudomonas sp. CYSI27, and Pseudomonas sp. TYRH42). The mesocosms were provided with nutrients (20 mg l− 1 N, 2.6 mg l− 1 P, and 16.4 mg l− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having C. laevigatus, bacteria, and NSA, and it is correlated with the copy numbers of alkB and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2197-4365 |
| Relation: | https://doaj.org/toc/2197-4365 |
| DOI: | 10.1186/s40643-024-00757-5 |
| Access URL: | https://doaj.org/article/5c8117426f664ceabe9176c0af2b7106 |
| Accession Number: | edsdoj.5c8117426f664ceabe9176c0af2b7106 |
| Database: | Directory of Open Access Journals |
| ISSN: | 21974365 |
|---|---|
| DOI: | 10.1186/s40643-024-00757-5 |
| Published in: | Bioresources and Bioprocessing |
| Language: | English |