Bibliographic Details
| Title: |
Quantification of cell energetics in human subcutaneous adipose progenitor cells after target gene knockdown |
| Authors: |
Liang Li, Anya M. Gunewardena, Tenzin Nyima, Brian J. Feldman |
| Source: |
STAR Protocols, Vol 4, Iss 4, Pp 102607- (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Cell Biology, Cell Culture, Cell Isolation, Metabolism, Science (General), Q1-390 |
| More Details: |
Summary: Pro-preadipocytes are adipocyte progenitor cells within subcutaneous adipose tissue that are conserved in human adipose tissue with distinct cellular energetics. Here, we detail a protocol to quantify cellular oxygen consumption rates of primary human cells harvested from adipose tissue. We describe steps for primary cell expansion, cell seeding, transfection, differentiation, and respirometry followed by Agilent Seahorse Analytics. The measurement of bioenergetic profiles and resulting data further expand our knowledge of the functional properties of primary cells isolated from adipose tissue.For complete details on the use and execution of this protocol, please refer to Chen et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166723005749; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102607 |
| Access URL: |
https://doaj.org/article/5b92283f95ed4391bc63a881baa9be27 |
| Accession Number: |
edsdoj.5b92283f95ed4391bc63a881baa9be27 |
| Database: |
Directory of Open Access Journals |
