Bibliographic Details
| Title: |
Preparation and Properties of Lysozyme Self-assembled Nanofibrils Films |
| Authors: |
Suhua YANG, Linxuan JIN, Chenyu NIU, Lingling LIU, Haobin CHEN, Yixing ZHU, Zhaojun BAN |
| Source: |
Shipin gongye ke-ji, Vol 44, Iss 21, Pp 251-257 (2023) |
| Publisher Information: |
The editorial department of Science and Technology of Food Industry, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Food processing and manufacture |
| Subject Terms: |
lysozyme, amyloid fibrosis, film, bacteriostatic effect, structure characterization, performance analysis, Food processing and manufacture, TP368-456 |
| More Details: |
This study exploited the use of lysozyme (LYZ) as a material to generate amyloid fibrosis process tuned by acid-heat induction under pH2.0 and 90 ℃. The structural characteristics of the lysozyme amyloid fibrils (A-LYZ) were characterized by nanoparticle size analyzer, atomic force microscopy (AFM), circular dichroism (CD), and fluorescence spectrophotometer. The four performance indicators on original lysozyme film and lysozyme amyloid fibrils film prepared by the flow-casting method, which were tensile strength (TS), elongation at break (E), water vapor permeability (WVP) and water solubility (WS), were inspected. Furthermore, the vanillin was added to lysozyme solution to obtain an antibacterial film, and its inhibitory effects on E. coli and B. subtilis were investigated. The results showed that the average particle size of lysozyme after amyloid fibrosis increased significantly, the structures of proteins changing from spherical to linear were confirmed by atomic force microscopy (AFM). Circular dichroism (CD) showed that the protein secondary structure was changed from α-helix to β-sheet. After acid and heat treatment, the surface structure of A-LYZ films was uniform and smooth. The mechanical properties of the films were increased and the WVP and WS of the films were decreased significantly (P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1002-0306 |
| Relation: |
https://doaj.org/toc/1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2022120218 |
| Access URL: |
https://doaj.org/article/a5b7b4a6124645b8984c54f296661cd9 |
| Accession Number: |
edsdoj.5b7b4a6124645b8984c54f296661cd9 |
| Database: |
Directory of Open Access Journals |
