Academic Journal
Mitochondrial superoxide contributes to oxidative stress exacerbated by DNA damage response in RAD51-depleted ovarian cancer cells
| Title: | Mitochondrial superoxide contributes to oxidative stress exacerbated by DNA damage response in RAD51-depleted ovarian cancer cells |
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| Authors: | Limei Xu, Tingting Wu, Shihua Lu, Xiaohe Hao, Junchao Qin, Jing Wang, Xiyu Zhang, Qiao Liu, Beihua Kong, Yaoqin Gong, Zhaojian Liu, Changshun Shao |
| Source: | Redox Biology, Vol 36, Iss , Pp 101604- (2020) |
| Publisher Information: | Elsevier, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine (General) LCC:Biology (General) |
| Subject Terms: | RAD51, Redox homeostasis, G2/M arrest, CHK1, Mitochondria stress, Ovarian cancer, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| More Details: | Ovarian cancer is the most lethal gynecological malignancy. Abnormal homologous recombination repair, high level of reactive oxygen species (ROS) and upregulation of antioxidant genes are characteristic features of ovarian cancer. However, the molecular mechanisms governing the redox homeostasis in ovarian cancer cells remain to be fully elucidated. We here demonstrated a critical role of RAD51, a protein essential for homologous recombination, in the maintenance of redox homeostasis. We found that RAD51 is overexpressed in high grade serous ovarian cancer and is associated with poor prognosis. Depletion or inhibition of RAD51 results in G2/M arrest, increased production of reactive oxygen species and accumulation of oxidative DNA damage. Importantly, antioxidant N-acetylcysteine (NAC) significantly attenuated the induction of DNA damage and the perturbation of proliferation caused by RAD51 depletion. We further demonstrated that RAD51 inhibition or depletion led to elevated production of mitochondrial superoxide and increased accumulation of mitochondria. Moreover, CHK1 activation is required for the G2/M arrest and the generation of mitochondrial stress in response to RAD51 depletion. Together, our results indicate that nuclear DNA damage caused by RAD51 depletion may trigger mitochondria-originated redox dysregulation. Our findings suggest that a vicious cycle of nuclear DNA damage, mitochondrial accumulation and oxidative stress may contribute to the tumor-suppressive effects of RAD51 depletion or inhibition. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2213-2317 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213231720308090; https://doaj.org/toc/2213-2317 |
| DOI: | 10.1016/j.redox.2020.101604 |
| Access URL: | https://doaj.org/article/5b50b962dc954c91b54c2a7b09e9c176 |
| Accession Number: | edsdoj.5b50b962dc954c91b54c2a7b09e9c176 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22132317 |
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| DOI: | 10.1016/j.redox.2020.101604 |
| Published in: | Redox Biology |
| Language: | English |