Development of new real‐time PCR methods for detection of Decapod iridescent virus 1 in shrimp
| Title: | Development of new real‐time PCR methods for detection of Decapod iridescent virus 1 in shrimp |
|---|---|
| Authors: | Melony J. Sellars, Louise Franz, Ralf Joachim Moser |
| Source: | Journal of the World Aquaculture Society, Vol 54, Iss 1, Pp 167-180 (2023) |
| Publisher Information: | Wiley, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Aquaculture. Fisheries. Angling |
| Subject Terms: | Decapod iridescent virus 1, Iridovirus, real‐time PCR, Shrimp MultiPath Xtra, Aquaculture. Fisheries. Angling, SH1-691 |
| More Details: | Abstract Novel Decapod Iridescent virus (DIV1) infections emerged in mainland China around 2014 and have devastated shrimp aquaculture operations in Chinese coastal provinces. In 2020, DIV1 has spread to Taiwan with devastating results to shrimp and crayfish farms, in addition to being found in wild caught Penaeus monodon from the Indian Ocean. This trend is a major cause for concern and an urgent reminder to expand the tools needed to monitor the spread of DIV1 globally. Here, we describe a set of four different real‐time polymerase chain reaction (PCR) assays positioned across the genome of DIV1 to detect the virus in shrimp tissues. All four assays show a wide dynamic range and high analytical sensitivity and specificity. In addition, the newly developed assays show excellent diagnostic sensitivity and specificity in clinical Litopenaeus vannamei samples of North Asian origin. The new molecular toolset will enhance global capabilities to monitor the spread of DIV1 and ultimately be used as an early warning system for farmers and authorities to engage in appropriate risk mitigation strategies. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1749-7345 0893-8849 |
| Relation: | https://doaj.org/toc/0893-8849; https://doaj.org/toc/1749-7345 |
| DOI: | 10.1111/jwas.12885 |
| Access URL: | https://doaj.org/article/e5ab73e9e621480a9fad8d5cc65b3920 |
| Accession Number: | edsdoj.5ab73e9e621480a9fad8d5cc65b3920 |
| Database: | Directory of Open Access Journals |
| ISSN: | 17497345 08938849 |
|---|---|
| DOI: | 10.1111/jwas.12885 |
| Published in: | Journal of the World Aquaculture Society |
| Language: | English |