Virulence gene profiling of porcine Pasteurella multocida isolates of Assam
| Title: | Virulence gene profiling of porcine Pasteurella multocida isolates of Assam |
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| Authors: | L. Babita Devi, Durlav Prasad Bora, S. K. Das, R. K. Sharma, S. Mukherjee, R. A. Hazarika |
| Source: | Veterinary World, Vol 11, Iss 3, Pp 348-354 (2018) |
| Publisher Information: | Veterinary World, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Animal culture LCC:Veterinary medicine |
| Subject Terms: | capsular type, Pasteurella multocida, porcine, virulence-associated genes, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: | Aim: The present study was conducted to detect and identify the virulence genes in Pasteurella multocida isolates of porcine origin from Assam. Materials and Methods: A total of 21 porcine P. multocida isolates were subjected to capsular typing and detection of virulence-associated genes (pfhA, tbpA, hgbB, toxA, oma87, ompH, and nanB) using various polymerase chain reaction (PCR) methods reported elsewhere. Further, pathogenicity of the porcine isolates of P. multocida was studied in mice. For each strain of P. multocida selected for pathogenicity trial, the group of mice was injected intraperitoneally (i/p) with 0.1 ml of the inoculum prepared from respective field isolates, containing 109 organisms per ml. Results: Capsular typing of the isolates by multiplex PCR showed two capsular types, type A (66.66%) and type D (33.33%). All the isolates were positive for outer membrane protein genes, oma87 and ompH genes. Iron acquisition genes, tbpA and hgbB, were detected in 14.28% and 19.04% of the isolates. The dermonecrotoxin encoding gene, toxA, was present in 23.80% of the isolates. Filamentous hemagglutinin encoding gene, pfhA, was detected in 28.57%. The virulence gene distribution pattern of the isolates indicates the important role of the genes in disease pathogenesis. Conclusion: From the present study, it can be concluded that toxA gene is an important marker gene for defining the pathogenic potential of P. multocida strains in swine. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0972-8988 2231-0916 |
| Relation: | http://www.veterinaryworld.org/Vol.11/March-2018/15.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2018.348-354 |
| Access URL: | https://doaj.org/article/dae5a74a9c1940e5be31107a21aafc22 |
| Accession Number: | edsdoj.5a74a9c1940e5be31107a21aafc22 |
| Database: | Directory of Open Access Journals |
| ISSN: | 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2018.348-354 |
| Published in: | Veterinary World |
| Language: | English |