Genomic characterization and pathogenicity of a novel fowl adenovirus serotype 11 isolated from chickens with inclusion body hepatitis in China

Bibliographic Details
Title: Genomic characterization and pathogenicity of a novel fowl adenovirus serotype 11 isolated from chickens with inclusion body hepatitis in China
Authors: Qilong Qiao, Minghe Xu, Xiangdong Wang, Jingge Tian, Yihang Zhang, Congcong Song, Junjie Liu, Yan Li, Xingyu Li, Panpan Yang, Mingzhen Song, Yongtao Li, Zeng Wang, Baiyu Wang, Jun Zhao
Source: Poultry Science, Vol 103, Iss 5, Pp 103642- (2024)
Publisher Information: Elsevier, 2024.
Publication Year: 2024
Collection: LCC:Animal culture
Subject Terms: fowl adenovirus serotype 11, inclusion body hepatitis, complete genome, pathogenicity, Animal culture, SF1-1100
More Details: ABSTRACT: Fowl adenovirus serotype 11 (FAdV-11) is one of the primary causative agents of inclusion body hepatitis (IBH), which causes substantial economic losses in the world poultry industry. In this study, we characterized the genome of the fowl adenovirus serotype 11 (FAdV-11) isolate FJSW/2021. The full genome of FJSW/2021 was 44, 154 base pairs (bp) in length and had a similar organization to that of previously reported FAdV-11 isolates. Notably, compared with those of other reported FAdV-11 strains, the preterminal protein (pTP) of FAdV-11 FJSW/2021 has six amino acid (aa) insertions (S-L-R-I-I-C) between 470 and 475 and one aa mutation of L476F; moreover, the tandem repeat (TR) regions of TR1 and TR2 were 33 bp (1 repeat) and 1,080 bp (8 repeats) shorter than those of the Canadian nonpathogenic isolate ON NP2, respectively. The pathogenicity of FJSW/2021 was studied in 10-day-old specific pathogen-free chicken embryos following allantoic cavity inoculation and in 1-day-old, 1-wk-old and 2-wk-old SPF chickens following intramuscular inoculation with 107 TCID50 of the virus. The results showed that FJSW/2021 can induce typical severe IBH in chicks less than 2 wk old. These findings highlighted the genetic differences between the pathogenic and non-pathogenic FAdV-11 isolates. The data will provide guidance for identifying the virulence factors of FAdV-11 strains. The animal challenge model developed in our study will allow precise evaluation of the efficacy of potential FAdV-11 vaccine candidates.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 0032-5791
Relation: http://www.sciencedirect.com/science/article/pii/S0032579124002219; https://doaj.org/toc/0032-5791
DOI: 10.1016/j.psj.2024.103642
Access URL: https://doaj.org/article/599c7c4aed01497a85970d2790c924ce
Accession Number: edsdoj.599c7c4aed01497a85970d2790c924ce
Database: Directory of Open Access Journals
More Details
ISSN:00325791
DOI:10.1016/j.psj.2024.103642
Published in:Poultry Science
Language:English