Identification of CKX gene family in Morus indica cv K2 and functional characterization of MiCKX4 during abiotic stress

Bibliographic Details
Title: Identification of CKX gene family in Morus indica cv K2 and functional characterization of MiCKX4 during abiotic stress
Authors: Chanchal Singhal, Arunima Singh, Arun Kumar Sharma, Paramjit Khurana
Source: Stress Biology, Vol 4, Iss 1, Pp 1-20 (2024)
Publisher Information: Springer, 2024.
Publication Year: 2024
Collection: LCC:Biology (General)
Subject Terms: Cytokinin oxidases, Phylogeny, Gene duplication, Expression analysis, Drought stress, Biology (General), QH301-705.5
More Details: Abstract Cytokinin oxidase/dehydrogenase (CKX) is the key enzyme that has been observed to catalyze irreversible inactivation of cytokinins and thus modulate cytokinin levels in plants. CKX gene family is known to have few members which are, expanded in the genome mainly due to duplication events. A total of nine MiCKXs were identified in Morus indica cv K2 with almost similar gene structures and conserved motifs and domains. The cis-elements along with expression analysis of these MiCKXs revealed their contrasting and specific role in plant development across different developmental stages. The localization of these enzymes in ER and Golgi bodies signifies their functional specification and property of getting modified post-translationally to carry out their activities. The overexpression of MiCKX4, an ortholog of AtCKX4, displayed longer primary root and higher number of lateral roots. Under ABA stress also the transgenic lines showed higher number of lateral roots and tolerance against drought stress as compared to wild-type plants. In this study, the CKX gene family members were analyzed bioinformatically for their roles under abiotic stresses.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2731-0450
Relation: https://doaj.org/toc/2731-0450
DOI: 10.1007/s44154-024-00173-x
Access URL: https://doaj.org/article/59852126c30e49ceae7d3c8f4683e1c1
Accession Number: edsdoj.59852126c30e49ceae7d3c8f4683e1c1
Database: Directory of Open Access Journals
More Details
ISSN:27310450
DOI:10.1007/s44154-024-00173-x
Published in:Stress Biology
Language:English