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Antifungal Activity of Bacillus velezensis X3-2 Against Plant Pathogens and Biocontrol Effect on Potato Late Blight

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Title: Antifungal Activity of Bacillus velezensis X3-2 Against Plant Pathogens and Biocontrol Effect on Potato Late Blight
Authors: Peixia Wei, Mengying Gao, Shuang Zhou, Guohui Liu, Pan Wang, Chunguang Liu, Fengshan Yang, Haiyan Fu
Source: Agriculture, Vol 14, Iss 12, p 2224 (2024)
Publisher Information: MDPI AG, 2024.
Publication Year: 2024
Collection: LCC:Agriculture (General)
Subject Terms: antimicrobial properties, potato, Phytophthora infestans, Bacillus velezensis, biocontrol, Agriculture (General), S1-972
More Details: Late blight of potato is caused by the pathogen Phytophthora infestans, which has been considered to be the most destructive disease affecting potato crops worldwide. In recent years, the use of antagonistic microorganisms to control potato late blight has become a green and environmentally friendly means of disease control, greatly reducing the use of chemical pesticides. To obtain antagonistic bacteria with a high biocontrol effect against potato late blight, a total of 16 antagonistic bacterial strains with an inhibition rate of more than 50% against P. infestans were screened from potato rhizosphere soil by double-culture method, among which the bacterial isolate (X3-2) had the strongest inhibitory activity against P. infestans, with an inhibition rate of 81.97 ± 4.81%, respectively, and a broad-spectrum inhibitory activity. The bacterial isolate (X3-2) was identified as Bacillus velezensis based on its 16S rDNA gene sequence and morphological as well as biochemical properties. The results of our in vitro experiments demonstrated that X3-2 was a potent inducer of resistance in potato tubers and leaflets against late blight. In greenhouse experiments, it was confirmed that the biological preparation X3-2 exhibits an anti-oomycete effect, demonstrating a significant control efficacy on potato late blight. Further analyses showed that the antagonistic substances of X3-2 were distributed both intracellularly and extracellularly. In addition, screening for plant-growth-promoting (PGP) traits showed that X3-2 has the ability to produce siderophores and secrete indole acetic acid (IAA). The findings from this research suggest that B. velezensis X3-2 exhibits promise as a biocontrol agent for managing late blight. In the future, the composition and mechanism of the action of its antimicrobial substances can be studied in depth, and field trials can be carried out to assess its actual prevention and control effects.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2077-0472
Relation: https://www.mdpi.com/2077-0472/14/12/2224; https://doaj.org/toc/2077-0472
DOI: 10.3390/agriculture14122224
Access URL: https://doaj.org/article/5892c8e8a1b0412893979433d58f76a4
Accession Number: edsdoj.5892c8e8a1b0412893979433d58f76a4
Database: Directory of Open Access Journals
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  Data: Antifungal Activity of Bacillus velezensis X3-2 Against Plant Pathogens and Biocontrol Effect on Potato Late Blight
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  Data: <searchLink fieldCode="AR" term="%22Peixia+Wei%22">Peixia Wei</searchLink><br /><searchLink fieldCode="AR" term="%22Mengying+Gao%22">Mengying Gao</searchLink><br /><searchLink fieldCode="AR" term="%22Shuang+Zhou%22">Shuang Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Guohui+Liu%22">Guohui Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Pan+Wang%22">Pan Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Chunguang+Liu%22">Chunguang Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Fengshan+Yang%22">Fengshan Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Haiyan+Fu%22">Haiyan Fu</searchLink>
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  Data: Agriculture, Vol 14, Iss 12, p 2224 (2024)
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  Data: <searchLink fieldCode="DE" term="%22antimicrobial+properties%22">antimicrobial properties</searchLink><br /><searchLink fieldCode="DE" term="%22potato%22">potato</searchLink><br /><searchLink fieldCode="DE" term="%22Phytophthora+infestans%22">Phytophthora infestans</searchLink><br /><searchLink fieldCode="DE" term="%22Bacillus+velezensis%22">Bacillus velezensis</searchLink><br /><searchLink fieldCode="DE" term="%22biocontrol%22">biocontrol</searchLink><br /><searchLink fieldCode="DE" term="%22Agriculture+%28General%29%22">Agriculture (General)</searchLink><br /><searchLink fieldCode="DE" term="%22S1-972%22">S1-972</searchLink>
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  Data: Late blight of potato is caused by the pathogen Phytophthora infestans, which has been considered to be the most destructive disease affecting potato crops worldwide. In recent years, the use of antagonistic microorganisms to control potato late blight has become a green and environmentally friendly means of disease control, greatly reducing the use of chemical pesticides. To obtain antagonistic bacteria with a high biocontrol effect against potato late blight, a total of 16 antagonistic bacterial strains with an inhibition rate of more than 50% against P. infestans were screened from potato rhizosphere soil by double-culture method, among which the bacterial isolate (X3-2) had the strongest inhibitory activity against P. infestans, with an inhibition rate of 81.97 ± 4.81%, respectively, and a broad-spectrum inhibitory activity. The bacterial isolate (X3-2) was identified as Bacillus velezensis based on its 16S rDNA gene sequence and morphological as well as biochemical properties. The results of our in vitro experiments demonstrated that X3-2 was a potent inducer of resistance in potato tubers and leaflets against late blight. In greenhouse experiments, it was confirmed that the biological preparation X3-2 exhibits an anti-oomycete effect, demonstrating a significant control efficacy on potato late blight. Further analyses showed that the antagonistic substances of X3-2 were distributed both intracellularly and extracellularly. In addition, screening for plant-growth-promoting (PGP) traits showed that X3-2 has the ability to produce siderophores and secrete indole acetic acid (IAA). The findings from this research suggest that B. velezensis X3-2 exhibits promise as a biocontrol agent for managing late blight. In the future, the composition and mechanism of the action of its antimicrobial substances can be studied in depth, and field trials can be carried out to assess its actual prevention and control effects.
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      – SubjectFull: Phytophthora infestans
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