Pro-angiogenic effects of Guo Min decoction in a zebrafish model
| Title: | Pro-angiogenic effects of Guo Min decoction in a zebrafish model |
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| Authors: | Ping-Hsun Lu, Sheng-Fen Tung, Chi-Chung Wen, Ching-Yuan Huang, Jhih-Wei Huang, Chih-Hsin Chen, Yau-Hung Chen |
| Source: | Tzu-Chi Medical Journal, Vol 37, Iss 1, Pp 58-64 (2025) |
| Publisher Information: | Wolters Kluwer Medknow Publications, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Medicine |
| Subject Terms: | angiogenesis, guo min decoction, real-time polymerase chain reaction, zebrafish, Medicine |
| More Details: | Objectives: Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD. Materials and Methods: In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model. Results: GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions. Conclusion: Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1016-3190 2223-8956 |
| Relation: | https://journals.lww.com/10.4103/tcmj.tcmj_160_24; https://doaj.org/toc/1016-3190; https://doaj.org/toc/2223-8956 |
| DOI: | 10.4103/tcmj.tcmj_160_24 |
| Access URL: | https://doaj.org/article/57e6d9df2a4348e8bd89b654a216a87f |
| Accession Number: | edsdoj.57e6d9df2a4348e8bd89b654a216a87f |
| Database: | Directory of Open Access Journals |
| ISSN: | 10163190 22238956 |
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| DOI: | 10.4103/tcmj.tcmj_160_24 |
| Published in: | Tzu-Chi Medical Journal |
| Language: | English |