Bibliographic Details
| Title: |
Noninvasive Evaluation of GIP Effects on β-Cell Mass Under High-Fat Diet |
| Authors: |
Sakura Kiyobayashi, Takaaki Murakami, Norio Harada, Hiroyuki Fujimoto, Yuki Murata, Naotaka Fujita, Keita Hamamatsu, Eri Ikeguchi-Ogura, Tomonobu Hatoko, Xuejing Lu, Shunsuke Yamane, Nobuya Inagaki |
| Source: |
Frontiers in Endocrinology, Vol 13 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Diseases of the endocrine glands. Clinical endocrinology |
| Subject Terms: |
beta cell mass, GIP, exendin, SPECT, high-fat diet, Diseases of the endocrine glands. Clinical endocrinology, RC648-665 |
| More Details: |
Pancreatic β-cell mass (BCM) has an importance in the pathophysiology of diabetes mellitus. Recently, glucagon-like peptide-1 receptor (GLP-1R)-targeted imaging has emerged as a promising tool for BCM evaluation. While glucose-dependent insulinotropic polypeptide/gastric inhibitory polypeptide (GIP) is known to be involved in high-fat diet (HFD)-induced obesity, the effect of GIP on BCM is still controversial. In this study, we investigated indium 111 (111In)-labeled exendin-4 derivative ([Lys12(111In-BnDTPA-Ahx)]exendin-4) single-photon emission computed tomography/computed tomography (SPECT/CT) as a tool for evaluation of longitudinal BCM changes in HFD-induced obese mice, at the same time we also investigated the effects of GIP on BCM in response to HFD using GIP-knockout (GIP-/-) mice. 111In-exendin-4 SPECT/CT was able to distinguish control-fat diet (CFD)-fed mice from HFD-fed mice and the pancreatic uptake values replicated the BCM measured by conventional histological methods. Furthermore, BCM expansions in HFD-fed mice were demonstrated by time-course changes of the pancreatic uptake values. Additionally, 111In-exendin-4 SPECT/CT demonstrated the distinct changes in BCM between HFD-fed GIP-/- (GIP-/-+HFD) and wild-type (WT+HFD) mice; the pancreatic uptake values of GIP-/-+HFD mice became significantly lower than those of WT+HFD mice. The different changes in the pancreatic uptake values between the two groups preceded those in fat accumulation and insulin resistance. Taken together with the finding of increased β-cell apoptosis in GIP-/-+HFD mice compared with WT+HFD mice, these data indicated that GIP has preferable effects on BCM under HFD. Therefore, 111In-exendin-4 SPECT/CT can be useful for evaluating increasing BCM and the role of GIP in BCM changes under HFD conditions. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-2392 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fendo.2022.921125/full; https://doaj.org/toc/1664-2392 |
| DOI: |
10.3389/fendo.2022.921125 |
| Access URL: |
https://doaj.org/article/56eb90921da74ff78c9ad91627258319 |
| Accession Number: |
edsdoj.56eb90921da74ff78c9ad91627258319 |
| Database: |
Directory of Open Access Journals |
