Academic Journal
Limited Emergence of Salmonella enterica Serovar Infantis Variants with Reduced Phage Susceptibility in PhagoVet-Treated Broilers
| Title: | Limited Emergence of Salmonella enterica Serovar Infantis Variants with Reduced Phage Susceptibility in PhagoVet-Treated Broilers |
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| Authors: | Sandra Sevilla-Navarro, Jennifer Otero, Júlia López-Pérez, Jan Torres-Boncompte, Tiago Prucha, Maarten De Gussem, Daniela Silva, Julia Burgan, Pablo Catalá-Gregori, Pilar Cortés, Montserrat Llagostera |
| Source: | Animals, Vol 14, Iss 16, p 2352 (2024) |
| Publisher Information: | MDPI AG, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Veterinary medicine LCC:Zoology |
| Subject Terms: | Salmonella Infantis, broilers, bacteriophage, PhagoVet, reduced phage susceptibility, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| More Details: | Salmonella enterica serovar Infantis (S. Infantis) poses a growing issue in the poultry sector, with phage-based products emerging as a safe and effective control measure. This study investigated the emergence of reduced-phage-susceptibility variants (RPSV) of S. Infantis in PhagoVet-treated broilers, given that RPSV could undermine phage treatment efficacy. The bacteriophages in the PhagoVet product were characterized using transmission electron microscopy (TEM), genome sequencing, and infection profiling. Furthermore, two broiler trials were conducted: a challenge group (T1) and a challenge-and-treated group (T2). The S. Infantis infective dose was set at 104 and 106 colony-forming units (CFUs) per animal, with PhagoVet administration at 106 and 108 plaque-forming units (PFUs) per animal, in Trials 1 and 2, respectively. The results revealed that the four PhagoVet bacteriophages belonged to different genera. PhagoVet evidenced broad-spectrum efficacy against 271 strains representing 18 Salmonella serovars. In Trial 1, PhagoVet reduced bacterial counts in feces to nearly undetectable levels by day 42, with no RPSV detected. However, in Trial 2, three and five RPSVs were detected in feces and ceca, respectively. Consequently, PhagoVet demonstrated efficacy against S. Infantis in broilers, and the potential impact of RPSV is deemed unlikely to compromise its efficacy. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/14/16/2352; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani14162352 |
| Access URL: | https://doaj.org/article/56a23c5d21a74b1b9deadd627ee2ee71 |
| Accession Number: | edsdoj.56a23c5d21a74b1b9deadd627ee2ee71 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20762615 |
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| DOI: | 10.3390/ani14162352 |
| Published in: | Animals |
| Language: | English |