Bibliographic Details
| Title: |
Amelioration of human acute lymphoblastic leukemia (ALL) cells by ZnO-TiO2-Chitosan-Amygdalin nanocomposites |
| Authors: |
Abozer Y. Elderdery, Badr Alzahrani, Fehaid Alanazi, Siddiqa M.A. Hamza, Ahmed M.E. Elkhalifa, Abdulaziz H. Alhamidi, Abdulrahim A. Alabdulsalam, A. Mohamedain, Suresh S. Kumar, Pooi Ling Mok |
| Source: |
Arabian Journal of Chemistry, Vol 15, Iss 8, Pp 103999- (2022) |
| Publisher Information: |
Elsevier, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Chemistry |
| Subject Terms: |
Cytotoxicity, MOLT-4 cells, Anticancer activity, ZnO-TiO2-Chitosan-Amygdalin nanocomposites, Chemistry, QD1-999 |
| More Details: |
The present study aims to study the cytotoxicity of ZnO-TiO2-Chitosan-Amygdalin nanocomposites (ZnO-TiO2-Chitosan-Amygdalin) on T lymphoblast cancer cells (MOLT-4). In a study, nanocomposites containing 2.5 to 15 µg/ml MTT were screened for their anticancer activity. Its anticancer properties were significantly higher than those of other nanocomposites with an IC50 value of 10.34 µg/ml. We studied the mechanism of action for cytotoxic cell death by fluorescence microscopy using Acridine Orange/EtBr (AO/EtBr) and Rhodamine 123 staining procedures. Using DCFH-DA, ZnO-TiO2-Chitosan-Amygdalin nanocomposites were analyzed to determine ROS production. The change in apoptotic protein expression for the 24 h following treatment with MOLT-4 cells for Caspase-3, 8, and 9. Nanocomposites containing ZnO-TiO2-Chitosan-Amygdalin increased the number of early and late apoptotic cells in MOLT-4 cells. ZnO-TiO2-Chitosan-Amygdalin nanocomposites also enhanced mitochondrial apoptosis through Caspase cascade signaling. MOLT-4 cells phosphorylated Caspase cascade in response to ZnO-TiO2-Chitosan-Amygdalin nanocomposites. Compared to the control group, the cancer cells treated with ZnO-TiO2-Chitosan-Amygdalin nanocomposites significantly arrest the proliferation and induces cleavage of pro-apoptotic proteins which leads to apoptotic cell death. Accordingly, ZnO-TiO2-Chitosan-Amygdalin nanocomposites might be effective against T lymphoblast cancer. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1878-5352 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S187853522200315X; https://doaj.org/toc/1878-5352 |
| DOI: |
10.1016/j.arabjc.2022.103999 |
| Access URL: |
https://doaj.org/article/e568df53e43849d39996f1c1847fdaee |
| Accession Number: |
edsdoj.568df53e43849d39996f1c1847fdaee |
| Database: |
Directory of Open Access Journals |
