| Title: |
Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| Authors: |
Laura Millares, Sergi Pascual, Concepción Montón, Marian García-Núñez, Cristina Lalmolda, Rosa Faner, Carme Casadevall, Laia Setó, Silvia Capilla, Amàlia Moreno, Ady Angélica Castro-Acosta, Carlos José Alvarez-Martinez, Oriol Sibila, Germán Peces-Barba, Borja G. Cosio, Alvar Agustí, Joaquim Gea, Eduard Monsó |
| Source: |
BMC Pulmonary Medicine, Vol 19, Iss 1, Pp 1-9 (2019) |
| Publisher Information: |
BMC, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Diseases of the respiratory system |
| Subject Terms: |
Bacterial community, Diversity, Eosinophils, Exacerbations, Sputum, Stable COPD, Diseases of the respiratory system, RC705-779 |
| More Details: |
Abstract Background The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. Conclusion The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2466 |
| Relation: |
http://link.springer.com/article/10.1186/s12890-019-0867-x; https://doaj.org/toc/1471-2466 |
| DOI: |
10.1186/s12890-019-0867-x |
| Access URL: |
https://doaj.org/article/a568107fbe3c4613b078ece0b4c21c5a |
| Accession Number: |
edsdoj.568107fbe3c4613b078ece0b4c21c5a |
| Database: |
Directory of Open Access Journals |
