Bibliographic Details
| Title: |
Using Microfluidic Hepatic Spheroid Cultures to Assess Liver Toxicity of T-2 Mycotoxin |
| Authors: |
Mercedes Taroncher, Alan M. Gonzalez-Suarez, Kihak Gwon, Samuel Romero, Angel D. Reyes-Figueroa, Yelko Rodríguez-Carrasco, María-José Ruiz, Gulnaz Stybayeva, Alexander Revzin, Jose M. de Hoyos-Vega |
| Source: |
Cells, Vol 13, Iss 11, p 900 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Cytology |
| Subject Terms: |
T-2, HepG2 cells, microfluidic devices, spheroids, cytotoxicity, Cytology, QH573-671 |
| More Details: |
The Fusarium fungi is found in cereals and feedstuffs and may produce mycotoxins, which are secondary metabolites, such as the T-2 toxin (T-2). In this work, we explored the hepatotoxicity of T-2 using microfluidic 3D hepatic cultures. The objectives were: (i) exploring the benefits of microfluidic 3D cultures compared to conventional 3D cultures available commercially (Aggrewell plates), (ii) establishing 3D co-cultures of hepatic cells (HepG2) and stellate cells (LX2) and assessing T-2 exposure in this model, (iii) characterizing the induction of metabolizing enzymes, and (iv) evaluating inflammatory markers upon T-2 exposure in microfluidic hepatic cultures. Our results demonstrated that, in comparison to commercial (large-volume) 3D cultures, spheroids formed faster and were more functional in microfluidic devices. The viability and hepatic function decreased with increasing T-2 concentrations in both monoculture and co-cultures. The RT-PCR analysis revealed that exposure to T-2 upregulates the expression of multiple Phase I and Phase II hepatic enzymes. In addition, several pro- and anti-inflammatory proteins were increased in co-cultures after exposure to T-2. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/13/11/900; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells13110900 |
| Access URL: |
https://doaj.org/article/5653ff004457445fb78059f1878ab28b |
| Accession Number: |
edsdoj.5653ff004457445fb78059f1878ab28b |
| Database: |
Directory of Open Access Journals |
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