Bibliographic Details
| Title: |
Reprogramming Microbial CO2-Metabolizing Chassis With CRISPR-Cas Systems |
| Authors: |
Hai-Yan Yu, Shu-Guang Wang, Peng-Fei Xia |
| Source: |
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Biotechnology |
| Subject Terms: |
carbon dioxide, CRISPR, genome editing, cyanobacteria, acetogen, methanogen, Biotechnology, TP248.13-248.65 |
| More Details: |
Global warming is approaching an alarming level due to the anthropogenic emission of carbon dioxide (CO2). To overcome the challenge, the reliance on fossil fuels needs to be alleviated, and a significant amount of CO2 needs to be sequestrated from the atmosphere. In this endeavor, carbon-neutral and carbon-negative biotechnologies are promising ways. Especially, carbon-negative bioprocesses, based on the microbial CO2-metabolizing chassis, possess unique advantages in fixing CO2 directly for the production of fuels and value-added chemicals. In order to fully uncover the potential of CO2-metabolizing chassis, synthetic biology tools, such as CRISPR-Cas systems, have been developed and applied to engineer these microorganisms, revolutionizing carbon-negative biotechnology. Herein, we review the recent advances in the adaption of CRISPR-Cas systems, including CRISPR-Cas based genome editing and CRISPR interference/activation, in cyanobacteria, acetogens, and methanogens. We also envision future innovations via the implementation of rising CRISPR-Cas systems, such as base editing, prime editing, and transposon-mediated genome editing. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-4185 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fbioe.2022.897204/full; https://doaj.org/toc/2296-4185 |
| DOI: |
10.3389/fbioe.2022.897204 |
| Access URL: |
https://doaj.org/article/561f81233c92421a86968301bafef8d6 |
| Accession Number: |
edsdoj.561f81233c92421a86968301bafef8d6 |
| Database: |
Directory of Open Access Journals |
